|
| Status |
Public on Feb 17, 2017 |
| Title |
Seq-Well: A portable, low-cost platform for single-cell RNA-seq of low-input samples |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Single-cell RNA -seq can precisely resolve cellular states, but applying this method to low-input samples is challenging. Here, we present Seq-Well, a portable, low-cost platform for massively parallel single-cell RNA -seq. Barcoded mRNA capture beads and single cells are sealed in an array of subnanoliter wells using a semipermeable membrane, enabling efficient cell lysis and transcript capture. We characterize Seq-Well extensively and use it to profile thousands of primary human macrophages exposed to tuberculosis.
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| |
|
| Overall design |
Single-cell RNA sequencing experiments of HEK/3T3 mixing, PBMCs, and TB-exposed Macrophages.
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| |
|
| Contributor(s) |
Shalek AK, Hughes TK |
| Citation(s) |
28192419 |
| Submission date |
Dec 16, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Alex Shalek |
| Organization name |
Massachusetts Institute of Technology
|
| Department |
Chemistry
|
| Lab |
Shalek Lab
|
| Street address |
77 Masachusetts Ave
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
| GPL19415 |
Illumina NextSeq 500 (Homo sapiens; Mus musculus) |
|
| Samples (7)
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| Relations |
| BioProject |
PRJNA374551 |
| SRA |
SRP099447 |
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