|
| Status |
Public on Jul 31, 2017 |
| Title |
CITE-seq: Large scale simultaneous measuremnt of epitopes and transcriptomes in single cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
Recent high-throughput single-cell sequencing approaches have been transformative for understanding complex cell populations, but are unable to provide additional phenotypic information, such as protein levels of cell-surface markers. Using oligonucleotide-labeled antibodies, we integrate measurements of cellular proteins and transcriptomes into an efficient, sequencing-based readout of single cells. This method is compatible with existing single-cell sequencing approaches and will readily scale as the throughput of these methods increase.
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| |
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| Overall design |
Cord blood mononuclear cells were profiled by CITE-seq using a panel of 13 antibodies.
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| Contributor(s) |
Stoeckius M |
| Citation(s) |
35017482 |
| Submission date |
Jul 06, 2017 |
| Last update date |
Jan 28, 2022 |
| Contact name |
Marlon Stoeckius |
| E-mail(s) |
mstoeckius@nygenome.org
|
| Organization name |
New York Genome Center
|
| Department |
Innovation Lab
|
| Street address |
101 Avenue of the Americas
|
| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10013 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (12)
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|
| Relations |
| BioProject |
PRJNA393315 |
| SRA |
SRP111194 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE100866_CBMC_8K_13AB_10X-ADT_clr-transformed.csv.gz |
945.1 Kb |
(ftp)(http) |
CSV |
| GSE100866_CBMC_8K_13AB_10X-ADT_umi.csv.gz |
189.8 Kb |
(ftp)(http) |
CSV |
| GSE100866_CBMC_8K_13AB_10X-RNA_umi.csv.gz |
14.2 Mb |
(ftp)(http) |
CSV |
| GSE100866_CD8_merged-ADT_clr-transformed.csv.gz |
58.4 Kb |
(ftp)(http) |
CSV |
| GSE100866_CD8_merged-ADT_umi.csv.gz |
19.3 Kb |
(ftp)(http) |
CSV |
| GSE100866_CD8_merged-RNA_umi.csv.gz |
1.4 Mb |
(ftp)(http) |
CSV |
| GSE100866_PBMC_vs_flow_10X-ADT_clr-transformed.csv.gz |
466.9 Kb |
(ftp)(http) |
CSV |
| GSE100866_PBMC_vs_flow_10X-ADT_umi.csv.gz |
129.4 Kb |
(ftp)(http) |
CSV |
| GSE100866_PBMC_vs_flow_10X-RNA_umi.csv.gz |
10.1 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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