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| Status |
Public on Oct 22, 2018 |
| Title |
Dissecting the human liver cellular landscape by single cell RNA-seq reveals novel intrahepatic monocyte/ macrophage populations |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The liver is the largest solid organ in the body and is critical for metabolic and immune functions. However, surprisingly little is known about the cells that make up the human liver and its immune microenvironment. To address this, we mapped the cellular landscape in the human liver using single cell RNA sequencing. We carefully fractionated fragile, fresh hepatic tissue from human livers to obtain viable parenchymal and non-parenchymal cells. Our single cell transcriptomics map reveals 20 discrete cell populations, including a novel description of distinct monocyte/ macrophage populations in the human liver. We present a comprehensive view of the human liver at single cell resolution that outlines new characteristics of resident cells in the liver, and in particular provides a new map of the human hepatic immune microenvironment.
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| |
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| Overall design |
Primary liver samples from 5 patients were used for single cell RNA sequencing by 10X Genomics
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| |
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| Contributor(s) |
MacParland SA, Liu JC, Bader GD, McGilvray ID |
| Citation(s) |
30348985 |
| Submission date |
Jun 07, 2018 |
| Last update date |
Nov 12, 2021 |
| Contact name |
Jeff Liu |
| E-mail(s) |
jeff.liu@utoronto.ca
|
| Phone |
416-340-4800
|
| Organization name |
University Health Network
|
| Department |
Princess Margaret Cancer Center
|
| Lab |
Zadeh Lab
|
| Street address |
101 College Street, RM 4-601
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5G 1L7 |
| Country |
Canada |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA475114 |
| SRA |
SRP149989 |
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