|
| Status |
Public on Dec 04, 2018 |
| Title |
Single Cell RNA-sequencing of cell types isolated by FACS from normal human prostates |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Single-cell RNA-sequencing was conducted on specific cell types and heterogeneous viable cells (sorted by FACS) in order to determine a cellular atlas of the normal human prostate.
|
| |
|
| Overall design |
Fresh human prostate specimens were digested into single-cell suspensions for flow cytometry, populations of cells were subject to single-cell RNA-sequencing using the 10x Genomics platform.
|
| |
|
| Contributor(s) |
Strand DW, Henry GH, Vezina CM |
| Citation(s) |
30566875 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| K01 DK098277 |
Mechanisms of Fatty Acid Metabolism in Prostate Differentiation and Disease |
UT SOUTHWESTERN MEDICAL CENTER |
Douglas William Strand |
| R01 DK115477 |
Notch-mediated 5ARI resistance in human BPH |
UT SOUTHWESTERN MEDICAL CENTER |
Douglas William Strand |
| R03 DK110497 |
Interplay Between Stem Cells and Inflammation in Benign Prostatic Hyperplasia |
UT SOUTHWESTERN MEDICAL CENTER |
Douglas William Strand |
| U54 DK104310 |
Cellular and molecular mediators of fibrosis in the development of urinary tract dysfunction |
UNIVERSITY OF WISCONSIN MADISON |
WILLIAM A RICKE |
| R01 DK099328 |
Role of DNA methylation in prostate glandular development and urinary function |
UNIVERSITY OF WISCONSIN MADISON |
CHAD M. VEZINA |
| U01 DK110807 |
Molecular and fate maps of prostatic stroma |
UNIVERSITY OF WISCONSIN MADISON |
CHAD M. VEZINA |
| DP2 GM128203 |
Combinatorial Biology of Gene Regulation for Cellular Engineering |
UT SOUTHWESTERN MEDICAL CENTER |
Gary Chung Hon |
|
| Submission date |
Jul 19, 2018 |
| Last update date |
Feb 24, 2020 |
| Contact name |
Gervaise H. Henry |
| E-mail(s) |
gervaise.henry@utsouthwestern.edu
|
| Phone |
2146480497
|
| Organization name |
UT Southwestern Medical Center
|
| Department |
Urology
|
| Lab |
Strand Lab
|
| Street address |
5323 Harry Hines Blvd, Mail Code:9110
|
| City |
Dallas |
| State/province |
Texas |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (16)
|
|
| This SubSeries is part of SuperSeries: |
| GSE120716 |
A cellular anatomy of the normal adult human prostate and prostatic urethra |
|
| Relations |
| BioProject |
PRJNA482100 |
| SRA |
SRP154549 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE117403_D17_FACS_filtered_barcodes.tsv.gz |
127.7 Kb |
(ftp)(http) |
TSV |
| GSE117403_D17_FACS_filtered_genes.tsv.gz |
258.6 Kb |
(ftp)(http) |
TSV |
| GSE117403_D17_FACS_filtered_matrix.mtx.gz |
132.3 Mb |
(ftp)(http) |
MTX |
| GSE117403_D27_FACS_filtered_barcodes.tsv.gz |
157.9 Kb |
(ftp)(http) |
TSV |
| GSE117403_D27_FACS_filtered_genes.tsv.gz |
258.6 Kb |
(ftp)(http) |
TSV |
| GSE117403_D27_FACS_filtered_matrix.mtx.gz |
151.8 Mb |
(ftp)(http) |
MTX |
| GSE117403_Pd_filtered_barcodes.tsv.gz |
142.0 Kb |
(ftp)(http) |
TSV |
| GSE117403_Pd_filtered_genes.tsv.gz |
258.6 Kb |
(ftp)(http) |
TSV |
| GSE117403_Pd_filtered_matrix.mtx.gz |
169.7 Mb |
(ftp)(http) |
MTX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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