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Status |
Public on Aug 01, 2019 |
Title |
Functional cooperation of glycine synthase-reductase pathway with Wood-Ljungdahl pathway for autotrophic growth of Clostridium drakei |
Organism |
Clostridium drakei |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
The Wood-Ljungdahl pathway in acetogens converts C1 compounds, such as CO2 and CO, into acetyl-CoA. Similarly, the glycine synthase pathway assimilates C1 compounds into glycine. Partial glycine synthase genes are widely conserved in the Wood-Ljungdahl pathway gene cluster but functional relationship between the pathways in autotrophic condition remains unknown. To comprehend, we assembled Clostridium drakei genome (5.7-Mbp) with intact glycine synthase pathway and constructed a genome-scale metabolic model, iSL836, predicting increased metabolic flux rates of the Wood-Ljungdahl pathway and the glycine synthase-reductase associated reactions under autotrophic conditions. Along with the observation of significant transcriptional activation of genes in the pathways, surprisingly, 13C-labeling experiments and enzyme activity assays confirmed the strain synthesizes glycine and converts into acetyl-phosphate. This study suggests the Wood-Ljungdahl and the glycine synthase-reductase pathways convert CO2 into acetyl-CoA and acetyl-phosphate, respectively. In our knowledge, this is the first report on co-utilization of the pathways under autotrophic growth in acetogen.
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Overall design |
Profile of whole transcripts of Clostridium drakei generated by deep sequencing using Miseq
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Contributor(s) |
Song Y, Shin J, Jin S, Lee G, Lee J, Kang S, Lee J, Kim D, Lee E, Cho S, Kim S, Kim D, Cho B |
Citation(s) |
32170009, 33563990 |
Submission date |
Aug 14, 2018 |
Last update date |
Feb 22, 2021 |
Contact name |
Yoseb Song |
E-mail(s) |
yosebiback@gmail.com
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Organization name |
KAIST
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Street address |
291 Daehak-ro
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City |
Yuseong-gu |
State/province |
Daejeon |
ZIP/Postal code |
34141 |
Country |
South Korea |
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Platforms (1) |
GPL25449 |
Illumina MiSeq (Clostridium drakei) |
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Samples (4)
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Relations |
BioProject |
PRJNA485902 |
SRA |
SRP157895 |
Supplementary file |
Size |
Download |
File type/resource |
GSE118519_RNA_DESeq2.txt.gz |
41.4 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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