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| Status |
Public on Nov 20, 2019 |
| Title |
Noncoding regions are the main source of targetable tumor-specific antigens |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: Tumor-specific antigens (TSAs) represent ideal targets for cancer immunotherapy, but very few of them have been identified. Therefore, the goal of this study was to develop a novel approach, combining RNA-Sequencing and mass spectrometry, to enlarge the landscape of actionable TSAs in seven human primary samples, namely 4 B-ALL and 3 lung tumor biopsies.
Methods: We performed RNA-Sequencing on each primary tumor sample (unreplicated) with the Illumina HiSeq200. Using those RNA-Sequencing data to build a global cancer database for each sample, we performed a transcriptomic-informed mass spectrometry analysis of their MHC I-associated peptides to identify TSAs.
Results: We identified a total of 30 TSAs, 90% of which derived from allegedly non-coding regions and would have been missed by standard approaches. Moreover, most of these TSAs derived from non-mutated yet cancer-restricted transcripts that can be shared by multiple tumors.
Conclusions: In conclusion, the strategy reported herein is readily applicable to human tumors and should considerably enlarge the landscape of actionable TSAs.
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| Overall design |
Transcriptomic analysis of 2 human samples of thymic epithelial cells and 4 human samples of medullary thymic epithelial cells using Illumina Next seq 500.
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| Contributor(s) |
Hesnard L, Perreault C |
| Citation(s) |
30518613, 35367648 |
| Submission date |
Mar 05, 2019 |
| Last update date |
Jun 23, 2022 |
| Contact name |
Krystel Vincent |
| E-mail(s) |
krystel.vincent.1@gmail.com
|
| Organization name |
Institute for Research in Immunology and Cancer
|
| Lab |
Immunobiology - Claude Perreault
|
| Street address |
2950, chemin de la Polytechnique, Marcelle-Coutu Pavilion
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3T 1J4 |
| Country |
Canada |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA525590 |
| SRA |
SRP187513 |
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