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Series GSE130473 Query DataSets for GSE130473
Status Public on May 21, 2019
Title Single cell analysis of human fetal liver captures the transcriptional profile of hepatobiliary hybrid progenitors
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The liver parenchyma is composed of hepatocytes and bile duct epithelial cells (BECs). Controversy exists regarding the cellular origin of human liver parenchymal tissue generation during embryonic development, homeostasis or repair. Here we report the existence of a hepatobiliary hybrid progenitor (HHyP) population in human fetal liver using single-cell RNA sequencing. HHyPs are anatomically restricted to the ductal plate of fetal liver and maintain a unique transcriptional profile distinct from fetal hepatocytes, mature hepatocytes and mature BECs. In addition, molecular heterogenicity within the EpCAM+ population of freshly isolated fetal and adult human liver reveals diverse gene expression signatures of hepatic and biliary lineage potential. Finally, we FACS isolated fetal HHyPs and confirmed their hybrid progenitor phenotype in vivo. Our study suggests that hepatobiliary progenitor cells previously identified in mice also exist in humans, and can be distinguished from other parenchymal populations, including mature BECs, by distinct gene expression profiles.
 
Overall design Primary samples from 5 2nd trimester human fetal livers and 3 uninjured adult human livers for single cell RNA sequencing by Smartseq2.
 
Contributor(s) Segal JM, Wesche DJ, Rashid ST
Citation(s) 31350390
Submission date Apr 30, 2019
Last update date Aug 02, 2019
Contact name Joe M Segal
E-mail(s) Joe.segal8@gmail.com
Organization name Kings college London
Department Centre of Stem Cell and Regenerative Medicine
Lab Tamir Rashid
Street address 28 floor Tower wing, Great Maze pond
City London
ZIP/Postal code SE1 9RT
Country United Kingdom
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (1475)
GSM3739296 Human fetal liver (A1_102#2)
GSM3739297 Human fetal liver (A10_102#136)
GSM3739298 Human fetal liver (A11_102#153)
Relations
BioProject PRJNA540429
SRA SRP194241

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE130473_Series_count_matrix.csv.gz 9.3 Mb (ftp)(http) CSV
GSE130473_Series_feature_matrix.csv.gz 14.2 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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