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| Status |
Public on Apr 09, 2020 |
| Title |
Characterization of cochlear development at the single cell level |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The organ of Corti, located in the floor of the scala media of the cochlea, acts as the primary sensory transducer of sound in mammals. This remarkable structure comprises a highly diverse cellular mosaic that includes two unique types of mechanosensory hair cells and an undefined number of associated supporting cell types. All of these cells are believed to arise from a developmental equivalence group referred to as the prosensory domain that is similarly thought to arise from a proneurosensory population that develops in the anterior-ventral region of the otocyst. The results of both classical embryologic manipulations and modern molecular genetic experiments suggest that otocyst precursor cells proceed through several rounds of lineage restriction that progressively specify subsets of cells as prosensory cells and ultimately as either hair cells or supporting cells. However, the relatively small sizes of the otocyst and inner ear have hindered efforts to determine the full diversity of cell types within the mature cochlea and to identify the transitional cell types that exist during development. The recent development of droplet-based methods for isolation and subsequent transcriptional profiling of individual cells provides a potential method to address both of these challenges. Therefore, we dissected, dissociated and then captured over 27,000 epithelial cells from the developing cochlear duct at specific time points between E14 and P7.
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| Overall design |
We characterized the transcriptomic profiles of cochlear epithelium celltypes of E14, E16, P1, P7 wild type (WT) mice at a single cell resolution using 10x Chromium and Illumina sequencing platforms. Data contain over 27,000 single cells.
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| Contributor(s) |
Kelly MC, Kolla L, Kelley MW, Morell RJ |
| Citation(s) |
32404924 |
| Submission date |
Sep 11, 2019 |
| Last update date |
Nov 21, 2022 |
| Contact name |
Robert Morell |
| E-mail(s) |
morellr@nidcd.nih.gov
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| Organization name |
National Institute on Deafness and Other Communication Disorders
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| Street address |
35A Convent Drive, Room 1F-103
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA565059 |
| SRA |
SRP221409 |
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