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Status |
Public on Nov 01, 2019 |
Title |
Single-cell transcriptomics uncovers zonation of function in the mesenchyme during liver fibrosis |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We profile the transcriptomes of ~30,000 mouse single cells to deconvolve the hepatic mesenchyme in healthy and fibrotic liver at high resolution. We reveal spatial zonation of hepatic stellate cells across the liver lobule, designated portal vein-associated HSC and central vein-associated HSC, and uncover an equivalent functional zonation in a mouse model of centrilobular fibrosis. Our work illustrates the power of single-cell transcriptomics to resolve key collagen-producing cells driving liver fibrosis with high precision.
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Overall design |
Single-cell transcriptomic data (10x and SS2) are presented from both healthy mice and following carbon tetrachloride administration (acute (72h) and chronic (6wk) injury models). SS2 data also contains cells harvested from mice 14d post bile duct ligation.
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Contributor(s) |
Dobie R, Henderson NC, Wilson-Kanamori JR |
Citation(s) |
31722201 |
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Submission date |
Sep 19, 2019 |
Last update date |
Nov 25, 2019 |
Contact name |
John Roger Wilson-Kanamori |
Organization name |
University of Edinburgh
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Department |
Centre for Inflammation Research
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Lab |
Henderson
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Street address |
47 Little France Crescent
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City |
Edinburgh |
ZIP/Postal code |
EH16 4TJ |
Country |
United Kingdom |
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Platforms (1) |
GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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Samples (11)
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Relations |
BioProject |
PRJNA566389 |
SRA |
SRP222529 |