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| Status |
Public on Sep 16, 2020 |
| Title |
High spatial resolution multi-omics atlas sequencing of mouse embryos |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Microfluidic deterministic barcoding of mRNAs and proteins in tissue slides followed by high throughput sequencing enables the construction of high-spatial-resolution multi-omics atlas at the genome scale. Applying it to mouse embryo tissues revealed major tissue (sub)types in early-stage organogenesis, brain micro-vasculatures, and the fine structure of an optical vesicle at the single-cell-layer resolution.
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| Overall design |
Applying new approach – microfluidic Deterministic Barcoding in Tissue for spatial omics sequencing to mouse embryo tissues in different resolution (10μm, 25μm, 50μm).
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| |
|
| Contributor(s) |
Fan R, Liu Y, Yang M, Deng Y |
| Citation(s) |
33188776 |
| Submission date |
Sep 25, 2019 |
| Last update date |
Dec 16, 2020 |
| Contact name |
Mingyu Yang |
| E-mail(s) |
mingyu.yang@yale.edu
|
| Phone |
2033618885
|
| Organization name |
Yale
|
| Department |
Biomedical Engineering
|
| Lab |
Fan group
|
| Street address |
55 Prospect St., Room 103
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06511 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA574105 |
| SRA |
SRP223246 |
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