|
| Status |
Public on Mar 15, 2021 |
| Title |
Discovery of tumor specific antigens in acute myeloid leukemia. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We performed RNA-Seq and mass spectrometry (MS) on the immunopeptidome of 19 human primary AML samples and discovered a set of 58 tumor specific antigens (TSA) that could serve to design an anti-AML immunotherapy.
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| Overall design |
Four proteogenomic approaches were used to create cancer-specific MS databases. Peptide identifications were made on the samples used for RNA-Seq and database construction. Various bioinformatic approaches were then performed to establish the TSA nature of the peptides identified by MS. Data reported here are paired-end stranded RNA-sequencing (R1 map on reverse strand and R2 on forward strand) of the 19 AML samples at a coverage >120M reads / sample. MS data are freely available on PRIDE (PXD018542 and 10.6019/PXD018542).
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| |
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| Contributor(s) |
Ehx G, Perreault C |
| Citation(s) |
33740418 |
| Submission date |
Mar 25, 2020 |
| Last update date |
Jun 14, 2021 |
| Contact name |
Claude Perreault |
| E-mail(s) |
claude.perreault@umontreal.ca
|
| Organization name |
Institute for Research in Immunology and Cancer
|
| Street address |
2950, Chemin de Polytechnique
|
| City |
Montréal |
| State/province |
Québec |
| ZIP/Postal code |
H3C 3J7 |
| Country |
Canada |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (19)
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| Relations |
| BioProject |
PRJNA615163 |
| SRA |
SRP254014 |
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