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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 15, 2020 |
| Title |
GM-CSF-producing T helper cells: a distinct subset of T helper cells that acquire Th1 phenotype during the CNS inflammation. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Using scRNA-seq and CyTOF, we identified eight populations of antigen-experienced CD45RA-CD4+ T cells in blood of healthy donors, including a population of GM-CSF-producing Th cells (ThGM) that lacked expression of signature transcription factors and cytokines of established Th lineages. In addition to GM-CSF, human and mouse ThGM cells also expressed IL-2, TNF, IL-3 and CCL20. ThGM cells maintained their phenotype through several cycles of activation, but upregulated expression of T-bet and IFN-g upon exposure to IL-12 in vitro, and in the CNS of mice with autoimmune neuroinflammation. ThGM cells were encephalitogenic; T-bet was not required for their development, but it was required for their encephalitogenicity. These findings demonstrate that ThGM cells constitute a distinct population of Th cells with lineage characteristics.
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| Overall design |
5 scRNAseq datasets were analyzed.
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| Contributor(s) |
Rasouli J, Casella G, Yoshimura S, Zhang W, Xiao D, Garifallou J, Gonzalez M, Wiedeman A, Mari ER, Hakonarson H, Long SA, Zhang G, Ciric B, Rostami A |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 08, 2020 |
| Last update date |
Sep 17, 2020 |
| Contact name |
James Garifallou |
| Organization name |
Children's Hospital of Philadelphia
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| Department |
Neonatology
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| Street address |
3615 Civic Center Boulevard, ARC1014
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| City |
Philadelphia |
| State/province |
Pennsylvania |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA631238 |
| SRA |
SRP260798 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE150132_RAW.tar |
422.2 Mb |
(http)(custom) |
TAR (of MTX, TSV) |
| GSE150132_aggregation_s1-5_barcodes.tsv.gz |
355.4 Kb |
(ftp)(http) |
TSV |
| GSE150132_aggregation_s1-5_features.tsv.gz |
297.6 Kb |
(ftp)(http) |
TSV |
| GSE150132_aggregation_s1-5_matrix.mtx.gz |
400.5 Mb |
(ftp)(http) |
MTX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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