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| Status |
Public on Apr 19, 2022 |
| Title |
Single Cell Transcriptomics of Human Native Kidney and Kidney Allografts |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We tested the hypothesis that single-cell RNA-sequencing (scRNA-seq, 10X) and computational analysis of human kidney allograft biopsies will reveal new cell types and cell states and yield insights to personalize the care of transplant recipients.
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| Overall design |
3 kidney biopsies from 3 individuals for scRNA-seq; (i) HK: native kidney biopsy from a living kidney, (ii) AK1: allograft kidney biopsy with transplant glomerulopathy, fibrosis, and worsening kidney function but with undetectable circulating anti-HLA antibodies, and (iii) AK2: allograft kidney biopsy after treatment of active antibody-mediated rejection but with persistent circulating donor-specific anti-HLA antibodies.
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| Contributor(s) |
Muthukumar T, Suryawanshi H, Morozov P |
| Citation(s) |
35657798 |
| Submission date |
Jun 02, 2020 |
| Last update date |
Jun 23, 2022 |
| Contact name |
Pavel Morozov |
| Organization name |
Rockefeller University
|
| Department |
Tuschl Lab
|
| Lab |
RNA biology Lab.
|
| Street address |
1230
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA636807 |
| SRA |
SRP265651 |
