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Status |
Public on Jul 30, 2020 |
Title |
Expression data from embryonic mouse spinal cords from NFIX knockout and wild-type mice. |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
The generation of cellular identity and diversity within the developing spinal cord is critically dependent on networks of gene expression controlled by transcription factors, such as Nuclear Factor One X (NFIX). NFIX has been identified as an important factor in promoting astrocyte formation during embryonic mouse spinal cord development. To gain a more comprehensive understanding of the transcriptional landscape controlled by NFIX within the developing spinal cord, here we performed microarray analysis on E14.5 wild-type and Nfix-/- mouse spinal cords, the age at which the expression of NFIX by neural progenitor cells lining the spinal central canal is strongest.
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Overall design |
Whole spinal cords from Nfix-/- mouse embryos at E14.5 (and wild-type littermate controls; n=4) were dissected for RNA extraction and hybridization on an Affymetrix Mouse Transcriptome Analysis microarray.
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Contributor(s) |
Matuzelski E, Piper M |
Citation(s) |
32938475 |
Submission date |
Jul 29, 2020 |
Last update date |
Oct 02, 2020 |
Contact name |
Michael Piper |
E-mail(s) |
m.piper@uq.edu.au
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Organization name |
University of Queensland
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Department |
School of Biomedical Science
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Street address |
Building 81, University of Queensland, St Lucia
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City |
Brisbane |
State/province |
QLD |
ZIP/Postal code |
4067 |
Country |
Australia |
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Platforms (1) |
GPL20775 |
[MTA-1_0] Affymetrix Mouse Transcriptome Array 1.0 [transcript (gene) CSV version] |
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Samples (8)
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GSM4699179 |
spinal cord at E14_Nfix-/-_biological rep 1 |
GSM4699180 |
spinal cord at E14_Nfix-/-_biological rep 2 |
GSM4699181 |
spinal cord at E14_Nfix-/-_biological rep 3 |
GSM4699182 |
spinal cord at E14_Nfix-/-_biological rep 4 |
GSM4699183 |
spinal cord at E14_Nfix+/+_biological rep 1 |
GSM4699184 |
spinal cord at E14_Nfix+/+_biological rep 2 |
GSM4699185 |
spinal cord at E14_Nfix+/+_biological rep 3 |
GSM4699186 |
spinal cord at E14_Nfix+/+_biological rep 4 |
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Relations |
BioProject |
PRJNA649417 |