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| Status |
Public on Oct 08, 2020 |
| Title |
Circulating tRNA-derived small RNAs (tsRNAs) signature for the diagnosis and prognosis of breast cancer |
| Organism |
Homo sapiens |
| Experiment type |
Other
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Liquid biopsy is noninvasive and convenient to detect cancer-derived materials in blood or other body fluids. The aim of this study was to identify tRNA-derived small RNAs (tsRNAs) in plasma that could distinguish patients with breast cancer (BC) from healthy controls. Basing on high-throughput sequencing, 15 significantly upregulated tsRNAs were selected and assessed in cell supernatants and cell lines. 6 tsRNAs were identified and verified in a large cohort of 120 patients with BC and 112 healthy controls. tRF-Arg-CCT-017, tRF-Gly-CCC-001 and tiRNA-Phe-GAA-003 could serve as novel diagnostic biomarkers. Meanwhile, tRF-Arg-CCT-017 and tiRNA-Phe-GAA-003 could also act as prognostic biomarkers. Target genes of these tsRNAs were related to the development of cancer. These results suggest that specific tsRNAs in plasma might serve as diagnostic and prognostic biomarkers of BC.
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| Overall design |
Expression profiling of tsRNAs in plasma samples of patiens with breast cancer
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| |
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| Contributor(s) |
Wang J, Ma G, Ge H, Han X, Mao X, Wang X, Veeramootoo JS, Xia T, Liu X, Wang S |
| Citation(s) |
33402674 |
| Submission date |
Oct 02, 2020 |
| Last update date |
Jan 19, 2021 |
| Contact name |
Shui Wang |
| E-mail(s) |
ws0801@hotmail.com
|
| Organization name |
The First Affiliated Hospital with Nanjing Medical University
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| Street address |
300 Guangzhou Road
|
| City |
Nanjing |
| State/province |
Jiangsu |
| ZIP/Postal code |
210029 |
| Country |
China |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA667069 |
| SRA |
SRP286192 |
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