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| Status |
Public on Aug 18, 2021 |
| Title |
mRNA-seq read counts of peripheral blood mononuclear cells from congenital generalized lipodystrophy patients and their gender/aged-matched controls |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Congenital generalized lipodystrophy (CGL) is an autosomal recessive disorder characterized by defective adipose tissue, extreme insulin resistance, and early onset of diabetes. There are four types of congenital generalized lipodystrophy based on the causative genetic alterations. The symptoms and the degrees of disease progression are varied among all affected individuals, which might be due to unknown genetic modifiers. To identify potential predictive biomarkers associated with the disease progressions, we recruited 7 patients (cgl2_p1,cgl2_p2,cgl2_p3,cgl2_p4,cgl2_p5,cgl2_p6,cgl2_p7),as well as gender/aged-matched controls (cgl2_c1,cgl2_c2,cgl2_c3,cgl2_c4,cgl2_c5,cgl2_c6,cgl2_c7).The total RNA samples were extracted from the participants' peripheral blood mononuclear cells, followed by RNA-Seq. To find the molecular signatures that might be associated with disease progression, patients were further categorized into sub-groups based on the results of biochemical analysis and their clinical symptoms (e.g. diabetes, metabolic syndrome and metal retardation). DEGs were identified for each sub-groups of patients, and pathway analysis was performed to explore the underlying dysregulated mechanisms.
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| Overall design |
PBMC from 7 patients and 7 gender/aged-matched controls were extracted total RNA, followed by RNA-Seq.
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| Contributor(s) |
Huang Y, Su T, Wang C, Wong S, Chien Y, Wang Y, Hwu W, Lee N |
| Citation(s) |
34645804 |
| BioProject |
PRJNA662421 |
| Submission date |
Oct 09, 2020 |
| Last update date |
Nov 03, 2021 |
| Contact name |
Yen-Hua Huang |
| E-mail(s) |
yhhuang@ym.edu.tw
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| Phone |
+886-2-28267982
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| Organization name |
National Yang-Ming University
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| Department |
Institute of Biomedical Informatics
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| Street address |
No. 155, Sec. 2, Li-Nong St., Beitou Dist
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| City |
Taipei City |
| ZIP/Postal code |
11221 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (14)
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| Relations |
| SRA |
SRP283451 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE159337_rare_dis_read_count_ori.csv.gz |
817.1 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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