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| Status |
Public on Jul 08, 2021 |
| Title |
Single cell RNA-seq of brain glioblastoma samples |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Glioblastomas (GBM) are the most aggressive type of brain tumors with a dismal prognosis. The effectiveness of anti-tumor drugs is thwarted by brain endothelial cells (ECs) in the tumor microenvironment. However, the characteristics of ECs remains poorly inventoried at the single-cell level. We performed single-cell RNA sequencing (scRNA-seq) for ECs from 4 GBMs and matching non-malignant brain samples, and identified distinct EC phenotypes in GBM.
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| Overall design |
We profiled freshly isolated cells from tumor core and paired surrounding peripheral tissue in 4 glioblastoma patients. Single-cell suspensions, magnetic-activated cell sorting (MACS) enriched for CD31+ cells, were subjected to scRNA-seq using a 10X genomics-based single-cell protocol.
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| Contributor(s) |
Zhang L, Yuan X, Dimberg A, Wang L, Betsholtz C |
| Citation(s) |
34228647 |
| Submission date |
Dec 03, 2020 |
| Last update date |
Jul 08, 2021 |
| Contact name |
L. H. |
| Organization name |
Uppsala University
|
| Street address |
Dag Hammarskjölds Väg 20
|
| City |
Uppsala |
| ZIP/Postal code |
751 85 |
| Country |
Sweden |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA682432 |
| SRA |
SRP295708 |
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