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| Status |
Public on Mar 31, 2021 |
| Title |
Transcriptomic analysis of loss of Gli1 in neural stem cells responding to demyelination in the mouse brain. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In the adult mammalian brain, Gli1 expressing neural stem cells reside in the subventricular zone and their progeny are recruited to sites of demyelination in the white matter where they regenerate oligodendrocytes, the myelin forming cells. Remarkably, genetic and pharmacologic inhibition of Gli1 enhances remyelination in the adult brain. To understand the molecular mechanisms involved, we performed a transcriptomic analysis to compare the expression of genes in Gli1 neural stem cells with either intact Gli1 expression or genetic loss of Gli1 from adult mice on control diet or cuprizone diet, which induces demyelination. These data will be a valuable resource for identifying therapeutic targets for enhancing remyelination in demyelinating diseases like multiple sclerosis.
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| Overall design |
Differential gene expression in fac-sorted fate-mapped Gli1 neural stem cells from the adult subventricular zone of Gli1-Het (Gli1 CreER/wt; Rgfp) and Gli1-null (Gli1 CreER/CreER; Rgfp) mice on control diet vs 0.2% cuprizone diet for 3 weeks. Total 12 samples were analyzed consisting of 3 replicates each from Gli1-Het mice on control diet, Gli1-Het mice on cuprizone diet, Gli1-Null mice on control diet and Gli1-Null mice on cuprizone diet.
Grant ID: DOH01-STEM5-2016-00305
Funding source: New York State DOH
Grant title: Mobilizing Neural Stem Cells for Remyelination
Name: James Salzer
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| Contributor(s) |
Samanta J, Salzer JL |
| Citation(s) |
34711861 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 NS100867 |
Role and Regulation of Neural Stem Cells in Remyelination |
NEW YORK UNIVERSITY SCHOOL OF MEDICINE |
JAMES SALZER |
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| Submission date |
Dec 04, 2020 |
| Last update date |
Nov 04, 2021 |
| Contact name |
James Salzer |
| E-mail(s) |
James.Salzer@nyulangone.org
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| Organization name |
New York University
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| Department |
Neuroscience
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| Street address |
435 East 30th street
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10016 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA682609 |
| SRA |
SRP295917 |
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