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| Status |
Public on Jun 11, 2021 |
| Title |
Identification of neural oscillations and epileptiform changes in human brain organoids |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In our study, we define neural network activities in human cortex+ganglionic eminence (GE) fusion orgaonids using a combination of calcium incator imaging and electrophysiology. We futher define abnormal network activities using fusion organoids generated from isogenic induced pluripotent stem cells containing or lacking MECP2 function derived from Rett syndrome patients. Single cell sequencing (10X genomics) was used to characterize the compositon of the fusion organoids used in our studies, and to define differentially expressed genes associated with MECP2 mutation.
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| Overall design |
Comparison of pools of organoids generated from isogenic control (WT) or MECP2 mutant (705delG/ E235fs; Mut) iPSC derived from a Rett syndrome patient, collected at 3 time points (Days 56, 70, and 100). Day 56 pools each contain 3 unfused Cortex and 3 unfused Ganglionic eminence organoids. Day 70 and 100 pools contain 3 Cortex+Gangiolnic eminence fusion organoids.
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| Contributor(s) |
Samarasinghe R, Buth JE, Novitch BG |
| Citation(s) |
34426698 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R00 HD096105 |
Human cerebral organoids as a model system for neural development and disease |
The Regents of the University of California |
Momoko Watanabe |
| K99 HD096105 |
Human cerebral organoids as a model system for neural development and disease |
UNIVERSITY OF CALIFORNIA LOS ANGELES |
Momoko Watanabe |
| K08 NS119747 |
Utilizing Human Brain Organoids to Model the Differential Effects of SCN8A Mutation on Cortex and Hippocampus |
UNIVERSITY OF CALIFORNIA LOS ANGELES |
RANMAL A SAMARASINGHE |
| R01 DA051897 |
Integrative Modeling of HIV-Associated Neurocognitive Disorder in Human Brain Organoids |
UNIVERSITY OF CALIFORNIA LOS ANGELES |
BENNETT G NOVITCH |
| R01 NS089817 |
Regulation of neural progenitor functions underlying cortical growth & complexity |
UNIVERSITY OF CALIFORNIA LOS ANGELES |
BENNETT G NOVITCH |
|
| Submission date |
Jan 26, 2021 |
| Last update date |
Mar 07, 2023 |
| Contact name |
Bennett Novitch |
| E-mail(s) |
bnovitch@ucla.edu, bnovitch@g.ucla.edu, bnovitch@mednet.ucla.edu
|
| Phone |
3107949339
|
| Organization name |
University of California, Los Angeles
|
| Department |
Department of Neurobiology
|
| Lab |
Novitch Lab
|
| Street address |
650 Charles E Young Dr. S, CHS 66-200
|
| City |
Los Angeles |
| State/province |
California |
| ZIP/Postal code |
90095 |
| Country |
USA |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA694995 |
| SRA |
SRP303362 |
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