 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Sep 13, 2021 |
| Title |
Single-cell RNA sequencing reveals markers of disease progression in primary cutaneous T-cell lymphoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Background: In early-stage mycosis fungoides (MF), the most common primary cutaneous T-cell lymphoma, limited skin involvement with patches and plaques is associated with a favorable prognosis. Nevertheless, approximately 20-30% of cases progress to tumors or erythroderma, resulting in poor outcome. At present, factors contributing to this switch from indolent to aggressive disease are only insufficiently understood.
Methods: In patients with advanced-stage MF, we compared patches with longstanding history to newly developed plaques and tumors by using single-cell RNA sequencing, and compared results with early-stage MF as well as nonlesional MF and healthy control skin.
Results: Despite considerable inter-individual variability, lesion progression was uniformly associated with downregulation of the tissue residency markers CXCR4 and CD69, the heat shock protein HSPA1A, the tumor suppressors and immunoregulatory mediators ZFP36 and TXNIP, and the interleukin 7 receptor (IL7R) within the malignant clone, but not in benign T cells. This phenomenon was not only found in conventional TCR-αβ MF, but also in a case of TCR-γδ MF, suggesting a common mechanism across MF subtypes. Conversely, malignant cells in clinically unaffected skin from MF patients showed upregulation of these markers.
Conclusions: Our data reveal a specific panel of biomarkers that might be used for monitoring MF disease progression. Altered expression of these genes may underlie the switch in clinical phenotype observed in advanced-stage MF.
|
| |
|
| Overall design |
Single-cell RNA-seq investigation of patch, plaque/tumor, and follow up lesions and nonlesional skin in CTCL patients.
------------------------------
Authors state "raw data are missing due to patient privacy concerns".
|
| |
|
| Contributor(s) |
Rindler K, Jonak C, Alkon N, Thaler FM, Kurz H, Shaw LE, Stingl G, Weninger W, Halbritter F, Bauer WM, Farlik M, Brunner PM |
| Citation(s) |
38565563 |
| Submission date |
Apr 23, 2021 |
| Last update date |
Apr 17, 2024 |
| Contact name |
Katharina Rindler |
| Organization name |
Medical University of Vienna
|
| Department |
Dermatology
|
| Street address |
Waehringer Guertel 18-20
|
| City |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (27)
|
|
| Relations |
| BioProject |
PRJNA724688 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE173205_RAW.tar |
1.0 Gb |
(http)(custom) |
TAR (of MTX, TSV) |
| Raw data not provided for this record |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...