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| Status |
Public on Aug 15, 2021 |
| Title |
Single-cell RNA-seq of bone marrow cells from aplastic anemia patient and healthy donor |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
BM samples were collected from two adult healthy donors and two AA patients at the first hospital affiliated from Zhejiang Chinese University. Mononuclear cells (MNCs) were isolated using Ficoll-Hypaque gradient separation. CD34+ cells were purified from MNCs with the human anti-CD34 MicroBeads Isolation kit according to the manufactures specifications. Isolated cell was the purification of CD34+ cell. Then, MNCs mixed with CD34+ cells at 4:1 ratio and were analyzed by 10×Genomics.
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| |
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| Overall design |
a large-scale single-cell transcriptomic sequencing of 20,000 bone marrow cells from two adult healthy donors and two AA patients
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| |
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| Contributor(s) |
Hu T, Zhao Y, Yu X, Gao R, Yin L |
| Citation(s) |
35046994 |
| Submission date |
Aug 12, 2021 |
| Last update date |
Jan 31, 2022 |
| Contact name |
Liming Yin |
| E-mail(s) |
yinlm0509@zcme.edu.cn
|
| Phone |
8613858010759
|
| Organization name |
Zhejiang Chinese Medical University
|
| Department |
Institute of hematology
|
| Street address |
548 Binwen Road
|
| City |
Hangzhou/Zhejiang Province |
| State/province |
Zhejiang Province |
| ZIP/Postal code |
310053 |
| Country |
China |
| |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (4)
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| Relations |
| BioProject |
PRJNA754149 |
| SRA |
SRP332279 |
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