|
| Status |
Public on Oct 30, 2009 |
| Title |
Expression data from human breast cancers pre and post chemothrapy |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Neoadjuvant chemotherapy has been shown to be equivalent to post-operative treatment for breast cancer, and allows for assessment of chemotherapy response. In a pilot trial of docetaxel (T) and capecitabine (X) neoadjuvant chemotherapy for Stage II/III BC, we assessed correlation between baseline gene expression and tumor response to treatment, and examined changes in gene expression associated with treatment. Patients received 4 cycles of TX. Tumor tissue obtained from Mammotome™ core biopsies pretreatment (BL) and post-Cycle 1 (C1) of TX was flash frozen and stored at -70C until processing. Gene expression analysis utilized Affymetrix HG-U133 Plus 2.0 GeneChip arrays. Statistical analysis was performed using BRB Array Tools after RMA normalization. Gene ontology (GO) pathway analysis used random variance t-tests with a significance level of p<0.005. For gene categories identified by GO pathway analysis as significant, expression levels of individual genes within those pathways were compared between classes using univariate t-tests; those genes with significance level of p<0.05 were reported.
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| Overall design |
The analysis of baseline gene expression included baseline specimens from twenty-eight patients: 11 responders and 17 non-responders. Analyses of changes in gene expression included paired baseline and post-cycle 1 specimens from 14 patients; of these 6 patients were responders and 8 patients were non-responders.
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| |
|
| Contributor(s) |
Korde LA, Lusa L |
| Citation(s) |
20012355 |
| Submission date |
Oct 26, 2009 |
| Last update date |
Jun 06, 2022 |
| Contact name |
Larissa Korde |
| Organization name |
University of Washington
|
| Department |
Division of Medical Oncology
|
| Street address |
825 Eastlake Ave, E G30630
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98119 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
| Samples (61)
|
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| Relations |
| BioProject |
PRJNA121631 |
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