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Series GSE193650 Query DataSets for GSE193650
Status Public on Jun 10, 2022
Title Identification of genes whose expression is affected by fuchi nashi pRNAi in early embryos of the spider Parasteatoda tepidariorum
Organism Parasteatoda tepidariorum
Experiment type Expression profiling by high throughput sequencing
Summary We identified a GATA-like gene named fuchi nashi (fuchi) as a gene required for establishing a germ disc in the early embryo of the spider Parasteatoda tepidariorum. fuchi transcript is initially expressed in cells at both polar regions of the embryo and later in endodermal and extraembryonic cells. To genome-widely identify genes whose expressions are regulated by fuchi, we conducted RNA-sequencing of whole embryos at three different early stages (stages 2 and 3 and early stage 5) that were untreated (control) or treated for fuchi parental RNA interference (pRNAi).
 
Overall design We extracted poly(A) mRNA from whole embryos at stage 2 (st2, 11 h after egg laying [AEL]), stage 3 (st3, 20 h AEL), and early stage 5 (st5e, 31 h AEL) that were prepared from the same mated females before and after treatment for fuchi pRNAi. The mRNAs were sequenced using the Illumina Miseq platform to identify differentiallly epxressed genes (DEGs) between the untreated and treated embryos for each stage. Two biological replicates were obtained for each sample type. We confirmed that the fuchi gene (aug3.g26874) itself was expectedly included in the detected DEGs for all the stages.
Web link https://www.brh2.jp/
 
Contributor(s) Iwasaki-Yokozawa S, Akiyama-Oda Y, Oda H
Citation(s) 36203191
Submission date Jan 13, 2022
Last update date Oct 24, 2022
Contact name Hiroki Oda
E-mail(s) hoda@brh.co.jp
Organization name JT Biohistory Research Hall
Lab Laboratory of Evolutionary Cell and Developmental Biology
Street address 1-1 Murasaki-cho
City Takatsuki
State/province Osaka
ZIP/Postal code 569-1125
Country Japan
 
Platforms (1)
GPL24841 Illumina MiSeq (Parasteatoda tepidariorum)
Samples (12)
GSM5815633 fuchi_st2_rep1_d00
GSM5815634 fuchi_st3_rep1_d00
GSM5815635 fuchi_st5e_rep1_d00
Relations
BioProject PRJNA797054

Download family Format
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MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE193650_HTseq_count_per_gene_aug3.txt.gz 1.0 Mb (ftp)(http) TXT
GSE193650_HTseq_count_per_gene_aug3_RPKM.txt.gz 1.9 Mb (ftp)(http) TXT
GSE193650_RAW.tar 1.7 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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