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| Status |
Public on Nov 04, 2022 |
| Title |
Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We present a single-cell perspective of healthy human kidney from 19 living donors, with equal contribution from males and females, profiling the transcriptome of 27677 high-quality cells to map healthy kidney at high resolution. Our sex-balanced dataset revealed sex-based differences in gene expression within proximal tubular cells, specifically, increased anti-oxidant metallothionein genes in females and the predominance of aerobic metabolism-related genes in males. Using CD45+ enrichment in 10 samples, we profile the immune niche of the kidney, identifying kidney-specific lymphocyte populations with unique transcriptional profiles indicative of kidney-adapted functions. We observed significant heterogeneity in resident myeloid populations and identified an MRC1+ LYVE1+ FOLR2+ C1QC+ population as the predominant myeloid population in healthy kidney. This study provides a broad cellular map of healthy human kidney, revealing novel insights into the complexity of renal parenchymal cells and kidney-resident immune populations.
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| Overall design |
Single -cell RNA sequencing (10X Genomics Single Cell 3’ v3) was performed on 19 core biopsies from human living donor kidneys, 9 biopsies from males and 10 from females. 10 samples (5 males, 5 females) were subjected to CD45-enrichment using magnetic EasySep Human CD45 depletion kit II (STEMCELL, cat# 17898).
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| Contributor(s) |
McEvoy CM, Murphy JM, Konvalinka A, Crome SQ |
| Citation(s) |
36496458 |
| Submission date |
May 03, 2022 |
| Last update date |
Dec 13, 2022 |
| Contact name |
Caitriona M McEvoy |
| E-mail(s) |
mcevoyc@gmail.com
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| Organization name |
University Health Network
|
| Street address |
200 Elizabeth St
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| City |
Toronto |
| ZIP/Postal code |
M5G 2C4 |
| Country |
Canada |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (19)
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| Relations |
| BioProject |
PRJNA834732 |
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