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| Status |
Public on Feb 13, 2023 |
| Title |
Single-cell RNAseq of the resident immune cells from Gli1HhipCKO_Tam and Gli1HhipCKO_Oil_Control mice lungs |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To study resident immune cells in Hhip-deleted lungs, we conduct single-cell RNAseq of the resident immune cells from Gli1HhipCKO_Tam and Gli1HhipCKO_Oil_Control mice lungs using 10X genomics scRNAseq technique.
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| |
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| Overall design |
Resident immune cells were sorted from adult control and Hhip-deleted lungs. Dead cells were excluded using DAPI negative selection.
|
| Web link |
https://doi.org/10.1016/j.immuni.2023.01.032
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| |
|
| Contributor(s) |
Wang C, Peng T |
| Citation(s) |
36822205 |
| Submission date |
Jun 22, 2022 |
| Last update date |
Feb 27, 2023 |
| Contact name |
Tien Peng |
| E-mail(s) |
tien.peng@ucsf.edu
|
| Organization name |
University of California San Francisco
|
| Department |
Department of Pulmonary Medicine
|
| Lab |
Tien Peng Lab
|
| Street address |
513 Parnassus Ave, Health Sciences East 1350
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (2) |
| GSM6261352 |
Oil-injected Gli1creERT/Hhipflo/flox |
| GSM6261353 |
Tamoxifen-injected Gli1creERT/Hhipflo/flox |
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| Relations |
| BioProject |
PRJNA851817 |
