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| Status |
Public on Aug 25, 2022 |
| Title |
Molecular and cellular changes underlying evolutionary specializations of primate dorsolateral prefrontal cortex II |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The granular dorsolateral prefrontal cortex (dlPFC) is an evolutionary specialization of primates that is centrally involved in cognition. Here, we assessed over 600,000 single-nucleus transcriptomes from human, chimpanzee, macaque, and marmoset dlPFC. While major subclasses of transcriptomically-defined cell subtypes are conserved, we detected several subtypes only in some species and substantial species-specific molecular differences across homologous neuronal, glial and non-neural subtypes. The latter are exemplified by human-specific switching between translation of the neuropeptide somatostatin (SST) and tyrosine hydroxylase (TH), the rate-limiting enzyme in dopamine production, in certain interneurons, and also by expression of the neuropsychiatric risk gene FOXP2, which is human-specific in microglia and primate-specific in layer-4 granular neurons. We generated a comprehensive transcriptomic and cellular survey of dlPFC evolution in anthropoid primates.
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| Overall design |
Single nucleus Multiome sequencing (ATAC-seq and RNA-seq) of the adult human dorsolateral prefrontal cortex
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| Contributor(s) |
Ma S, Skarica M, Girgenti M, Strittmatter SM, Sousa A, Sestan N |
| Citation(s) |
36007006 |
| Submission date |
Jul 01, 2022 |
| Last update date |
Nov 21, 2023 |
| Contact name |
Nenad Sestan |
| E-mail(s) |
nenad.sestan@yale.edu
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| Organization name |
Yale University
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| Department |
Neuroscience
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| Street address |
333 Cedar street
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| City |
New haven |
| State/province |
CT |
| ZIP/Postal code |
06510 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA854879 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE207334_Multiome_atac_counts.mtx.gz |
463.2 Mb |
(ftp)(http) |
MTX |
| GSE207334_Multiome_atac_peaks.txt.gz |
1.3 Mb |
(ftp)(http) |
TXT |
| GSE207334_Multiome_cell_meta.txt.gz |
3.5 Mb |
(ftp)(http) |
TXT |
| GSE207334_Multiome_rna_counts.mtx.gz |
424.5 Mb |
(ftp)(http) |
MTX |
| GSE207334_Multiome_rna_genes.txt.gz |
216.5 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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