The purpose is to obtain samples for mRNA analysis in HUV-EC-C cells, a human endothelial cell line isolated from the vein of the umbilical cord, using the following viruses obtained by reverse genetics: Zaire Ebola virus wild-type in the ΔVP30 background (deltaVP30-WT) and mutant lacking the mucin domain (deltaVP30-deltamucin).
Overall design
HUVEC cells were infected with a MOI=500 adenovirus VP30 overnight. 24 hours post adeno-infection cells were either Mock, deltaVP30-WT-EBOV (MOI=10), or deltaVP30-delta-mucin-EBOV (MOI=10) infected. Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Time points: 0, 8, 24, 48, and 72 h post-infection.
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