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| Status |
Public on Feb 28, 2011 |
| Title |
Clinical Significance of Osteoprotegerin Expression in Human Colorectal Cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Purpose: This study aimed to identify a novel biomarker or a target of treatment for colorectal cancer (CRC).
Experimental Design: The expression profiles of cancer cells in 104 patients with CRC were examined using laser microdissection and oligonucleotide microarray analysis. Overexpression in CRC cells especially in patients with distant metastasis was a prerequisite to select candidate genes. The mRNA expression of candidate gene was investigated by quantitative reversetranscription polymerase chain reaction (RT-PCR) in 77 patients as a validation study. We analyzed the protein expression and localization of the candidate gene by immunohistochemical study, and investigated the relationship between the expression and the clinicopathological feature in 274 CRC patients.
Results: We identified 6 genes as candidates related to distant metastasis in CRC patients by microarray analysis. Among these genes, Osteoprotegerin (OPG) is known to have an association with aggressiveness in several cancers through inhibiting apoptosis by neutralizing the function of tumor necrosis factor related apoptosis inducing ligand (TRAIL). The mRNA expression of OPG in cancer tissues was significantly higher in patients with distant metastasis than those without metastasis. The overexpression of OPG protein was significantly associated with worse overall survival and relapse free survival (RFS). Moreover, the overexpression of OPG protein was an independent risk factor for recurrence of CRC.
Conclusion: The overexpression of OPG would be a predictive biomarker of recurrence and a target of the treatment for CRC.
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| Overall design |
Total of 148 microarray datasets obtained from LCM and homogenized tissues of colorectal cancer patients were normalized using robust multi-array average (RMA) method under R 2.6.2 statistical software with affy package from BioConductor. Normalization was separately performed for LCM dataset and homogenized tissue dataset. The normalized gene expression levels were presented as log2-transformed values by RMA.
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| Contributor(s) |
Tsukamoto S, Ishikawa T, Iida S, Ishiguro M, Mogushi K, Mizushima H, Uetake H, Tanaka H, Sugihara K |
| Citation(s) |
21270110 |
| Submission date |
Apr 26, 2010 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Kaoru Mogushi |
| E-mail(s) |
mogushi-k@umin.ac.jp
|
| Phone |
+81-3-5802-1797
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| Organization name |
Juntendo University
|
| Department |
Intractable Disease Research Center
|
| Street address |
2-1-1 Hongo
|
| City |
Bunkyo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
113-8421 |
| Country |
Japan |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (148)
|
| GSM537330 |
patient 006, cancer, LCM [OPG study] |
| GSM537331 |
patient 100, cancer, LCM [OPG study] |
| GSM537332 |
patient 101, cancer, LCM [OPG study] |
| GSM537333 |
patient 102, cancer, LCM [OPG study] |
| GSM537334 |
patient 103, cancer, LCM [OPG study] |
| GSM537335 |
patient 104, cancer, LCM [OPG study] |
| GSM537336 |
patient 107, cancer, LCM [OPG study] |
| GSM537337 |
patient 108, cancer, LCM [OPG study] |
| GSM537338 |
patient 110, cancer, LCM [OPG study] |
| GSM537339 |
patient 111, cancer, LCM [OPG study] |
| GSM537340 |
patient 113, cancer, LCM [OPG study] |
| GSM537341 |
patient 115, cancer, LCM [OPG study] |
| GSM537342 |
patient 116, cancer, LCM [OPG study] |
| GSM537343 |
patient 117, cancer, LCM [OPG study] |
| GSM537344 |
patient 118, cancer, LCM [OPG study] |
| GSM537345 |
patient 119, cancer, LCM [OPG study] |
| GSM537346 |
patient 011, cancer, LCM [OPG study] |
| GSM537347 |
patient 123, cancer, LCM [OPG study] |
| GSM537348 |
patient 124, cancer, LCM [OPG study] |
| GSM537349 |
patient 125, cancer, LCM [OPG study] |
| GSM537350 |
patient 127, cancer, LCM [OPG study] |
| GSM537351 |
patient 128, cancer, LCM [OPG study] |
| GSM537352 |
patient 134, cancer, LCM [OPG study] |
| GSM537353 |
patient 135, cancer, LCM [OPG study] |
| GSM537354 |
patient 136, cancer, LCM [OPG study] |
| GSM537355 |
patient 138, cancer, LCM [OPG study] |
| GSM537356 |
patient 139, cancer, LCM [OPG study] |
| GSM537357 |
patient 142, cancer, LCM [OPG study] |
| GSM537358 |
patient 143, cancer, LCM [OPG study] |
| GSM537359 |
patient 144, cancer, LCM [OPG study] |
| GSM537360 |
patient 145, cancer, LCM [OPG study] |
| GSM537361 |
patient 147, cancer, LCM [OPG study] |
| GSM537362 |
patient 148, cancer, LCM [OPG study] |
| GSM537363 |
patient 150, cancer, LCM [OPG study] |
| GSM537364 |
patient 152, cancer, LCM [OPG study] |
| GSM537365 |
patient 153, cancer, LCM [OPG study] |
| GSM537366 |
patient 154, cancer, LCM [OPG study] |
| GSM537367 |
patient 155, cancer, LCM [OPG study] |
| GSM537368 |
patient 156, cancer, LCM [OPG study] |
| GSM537369 |
patient 015, cancer, LCM [OPG study] |
| GSM537370 |
patient 022, cancer, LCM [OPG study] |
| GSM537371 |
patient 023, cancer, LCM [OPG study] |
| GSM537372 |
patient 024, cancer, LCM [OPG study] |
| GSM537373 |
patient 025, cancer, LCM [OPG study] |
| GSM537374 |
patient 026, cancer, LCM [OPG study] |
| GSM537375 |
patient 030, cancer, LCM [OPG study] |
| GSM537376 |
patient 031, cancer, LCM [OPG study] |
| GSM537377 |
patient 033, cancer, LCM [OPG study] |
| GSM537378 |
patient 034, cancer, LCM [OPG study] |
| GSM537379 |
patient 036, cancer, LCM [OPG study] |
| GSM537380 |
patient 037, cancer, LCM [OPG study] |
| GSM537381 |
patient 038, cancer, LCM [OPG study] |
| GSM537382 |
patient 041, cancer, LCM [OPG study] |
| GSM537383 |
patient 046, cancer, LCM [OPG study] |
| GSM537384 |
patient 048, cancer, LCM [OPG study] |
| GSM537385 |
patient 053, cancer, LCM [OPG study] |
| GSM537386 |
patient 057, cancer, LCM [OPG study] |
| GSM537387 |
patient 061, cancer, LCM [OPG study] |
| GSM537388 |
patient 063, cancer, LCM [OPG study] |
| GSM537389 |
patient 068, cancer, LCM [OPG study] |
| GSM537390 |
patient 072, cancer, LCM [OPG study] |
| GSM537391 |
patient 073, cancer, LCM [OPG study] |
| GSM537392 |
patient 077, cancer, LCM [OPG study] |
| GSM537393 |
patient 078, cancer, LCM [OPG study] |
| GSM537394 |
patient 081, cancer, LCM [OPG study] |
| GSM537395 |
patient 082, cancer, LCM [OPG study] |
| GSM537396 |
patient 083, cancer, LCM [OPG study] |
| GSM537397 |
patient 088, cancer, LCM [OPG study] |
| GSM537398 |
patient 094, cancer, LCM [OPG study] |
| GSM537399 |
patient 095, cancer, LCM [OPG study] |
| GSM537400 |
patient 099, cancer, LCM [OPG study] |
| GSM537401 |
patient 106, cancer, LCM [OPG study] |
| GSM537402 |
patient 109, cancer, LCM [OPG study] |
| GSM537403 |
patient 121, cancer, LCM [OPG study] |
| GSM537404 |
patient 129, cancer, LCM [OPG study] |
| GSM537405 |
patient 130, cancer, LCM [OPG study] |
| GSM537406 |
patient 131, cancer, LCM [OPG study] |
| GSM537407 |
patient 133, cancer, LCM [OPG study] |
| GSM537408 |
patient 140, cancer, LCM [OPG study] |
| GSM537409 |
patient 141, cancer, LCM [OPG study] |
| GSM537410 |
patient 146, cancer, LCM [OPG study] |
| GSM537411 |
patient 149, cancer, LCM [OPG study] |
| GSM537412 |
patient 019, cancer, LCM [OPG study] |
| GSM537413 |
patient 027, cancer, LCM [OPG study] |
| GSM537414 |
patient 028, cancer, LCM [OPG study] |
| GSM537415 |
patient 032, cancer, LCM [OPG study] |
| GSM537416 |
patient 035, cancer, LCM [OPG study] |
| GSM537417 |
patient 042, cancer, LCM [OPG study] |
| GSM537418 |
patient 044, cancer, LCM [OPG study] |
| GSM537419 |
patient 045, cancer, LCM [OPG study] |
| GSM537420 |
patient 052, cancer, LCM [OPG study] |
| GSM537421 |
patient 055, cancer, LCM [OPG study] |
| GSM537422 |
patient 059, cancer, LCM [OPG study] |
| GSM537423 |
patient 064, cancer, LCM [OPG study] |
| GSM537424 |
patient 065, cancer, LCM [OPG study] |
| GSM537425 |
patient 070, cancer, LCM [OPG study] |
| GSM537426 |
patient 079, cancer, LCM [OPG study] |
| GSM537427 |
patient 084, cancer, LCM [OPG study] |
| GSM537428 |
patient 086, cancer, LCM [OPG study] |
| GSM537429 |
patient 089, cancer, LCM [OPG study] |
| GSM537430 |
patient 090, cancer, LCM [OPG study] |
| GSM537431 |
patient 096, cancer, LCM [OPG study] |
| GSM537432 |
patient 097, cancer, LCM [OPG study] |
| GSM537433 |
patient 098, cancer, LCM [OPG study] |
| GSM549099 |
patient 107, normal, homogenized [OPG study] |
| GSM549100 |
patient 110, normal, homogenized [OPG study] |
| GSM549101 |
patient 111, normal, homogenized [OPG study] |
| GSM549102 |
patient 115, normal, homogenized [OPG study] |
| GSM549103 |
patient 117, normal, homogenized [OPG study] |
| GSM549104 |
patient 119, normal, homogenized [OPG study] |
| GSM549105 |
patient 128, normal, homogenized [OPG study] |
| GSM549106 |
patient 134, normal, homogenized [OPG study] |
| GSM549107 |
patient 135, normal, homogenized [OPG study] |
| GSM549108 |
patient 138, normal, homogenized [OPG study] |
| GSM549109 |
patient 139, normal, homogenized [OPG study] |
| GSM549110 |
patient 046, normal, homogenized [OPG study] |
| GSM549111 |
patient 047, normal, homogenized [OPG study] |
| GSM549112 |
patient 048, normal, homogenized [OPG study] |
| GSM549113 |
patient 057, normal, homogenized [OPG study] |
| GSM549114 |
patient 063, normal, homogenized [OPG study] |
| GSM549115 |
patient 068, normal, homogenized [OPG study] |
| GSM549116 |
patient 072, normal, homogenized [OPG study] |
| GSM549117 |
patient 073, normal, homogenized [OPG study] |
| GSM549118 |
patient 074, normal, homogenized [OPG study] |
| GSM549119 |
patient 077, normal, homogenized [OPG study] |
| GSM549120 |
patient 078, normal, homogenized [OPG study] |
| GSM549121 |
patient 080, normal, homogenized [OPG study] |
| GSM549122 |
patient 082, normal, homogenized [OPG study] |
| GSM549123 |
patient 088, normal, homogenized [OPG study] |
| GSM549124 |
patient 006, cancer, homogenized [OPG study] |
| GSM549125 |
patient 011, cancer, homogenized [OPG study] |
| GSM549126 |
patient 015, cancer, homogenized [OPG study] |
| GSM549127 |
patient 024, cancer, homogenized [OPG study] |
| GSM549128 |
patient 025, cancer, homogenized [OPG study] |
| GSM549129 |
patient 027, cancer, homogenized [OPG study] |
| GSM549130 |
patient 028, cancer, homogenized [OPG study] |
| GSM549131 |
patient 030, cancer, homogenized [OPG study] |
| GSM549132 |
patient 031, cancer, homogenized [OPG study] |
| GSM549133 |
patient 032, cancer, homogenized [OPG study] |
| GSM549134 |
patient 033, cancer, homogenized [OPG study] |
| GSM549135 |
patient 035, cancer, homogenized [OPG study] |
| GSM549136 |
patient 036, cancer, homogenized [OPG study] |
| GSM549137 |
patient 038, cancer, homogenized [OPG study] |
| GSM549138 |
patient 041, cancer, homogenized [OPG study] |
| GSM549139 |
patient 042, cancer, homogenized [OPG study] |
| GSM549140 |
patient 044, cancer, homogenized [OPG study] |
| GSM549141 |
patient 045, cancer, homogenized [OPG study] |
| GSM549142 |
patient 047, cancer, homogenized [OPG study] |
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| Relations |
| BioProject |
PRJNA125983 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE21510_RAW.tar |
735.4 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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