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| Status |
Public on Dec 27, 2022 |
| Title |
Gene expression profile at single cell level of the intestinal T cells from patients with inflammatory bowel disease |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
T cells are key mediators of inflammation. Here we performed deep profiling of T cells in the gut of patients with inflammatory bowel disease to reveal the functional specialization of lymphocytes under pathological conditions and provide comprehensive insights into the pathogenesis of this disease.
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| Overall design |
Intestinal CD3+ T cells were isolated by Fluorescence-activated cell sorting (FACS) and analyzed using scRNAseq. This technology was further combined to BD AbSeq system to measure protein and transcriptome levels simultaneously at a single cell level.
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| Contributor(s) |
Yokoi T, Murakami M, Okuzaki D, Motooka D, Takeda K |
| Citation(s) |
36574662 |
| Submission date |
Nov 15, 2022 |
| Last update date |
Jul 18, 2023 |
| Contact name |
Daisuke Motooka |
| Organization name |
Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University
|
| Street address |
3-1, Yamadaoka
|
| City |
Suita |
| State/province |
Osaka |
| ZIP/Postal code |
5650871 |
| Country |
Japan |
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| Platforms (2) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA901899 |
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