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| Status |
Public on Jun 18, 2015 |
| Title |
IHH microRNA response to genetically-reconstructed Zaire Ebola viruses [miRNA] |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
The purpose of this experiment was to obtain samples for mRNA analysis in IHH cells infected with Zaire Ebola virus and mutants:
Zaire Ebola virus: This wild-type Ebola virus - strain Mayinga - was isolated from a fatal human case in Zaire (now known as the Democratic Republic of Congo) in 1976
Zaire Ebola virus, VP35 R312A possesses a R312A mutation in the VP35 protein.
Zaire Ebola virus, delta sGP. Lacks the ability to produce non-structural protein, the secreted glycoprotein (sGP).
Zaire Ebola virus, delta mucin. Lacks the mucin-like domain (MLD), which contains both N-linked and O-linked glycosylation sites, for the glycoproteins.
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| Overall design |
Overview of Experiment: Cells: Immortalized Human Hepatocytes (IHH); seed 60,000 cells per well in a 24-well plate. Infected with a multiplicity of infection (MOI) of 0.5. After infection, 3x wash with PBS and replace with 5% FCS DMEM without NaPyr or NEAA. Time matched mocks done in triplicate from same cell stock as rest of samples. Time Points = 0, 6, 12, 24, 48, and 72 hrs post infection in triplicate.
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| Contributor(s) |
Kawaoka Y, Waters KM, Halfmann P, Eisfeld AJ |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jun 17, 2015 |
| Last update date |
Aug 31, 2016 |
| Contact name |
Natalie Heller |
| E-mail(s) |
natalie.heller@pnnl.gov
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| Organization name |
PNNL
|
| Street address |
902 Battelle Blvd.
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| City |
Richland |
| ZIP/Postal code |
99354 |
| Country |
USA |
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| Platforms (1) |
| GPL19730 |
Agilent-046064 Unrestricted_Human_miRNA_V19.0_Microarray (Probe Name version) |
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| Samples (15)
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| This SubSeries is part of SuperSeries: |
| GSE65575 |
Modeling Host Responses to Understand Severe Human Virus Infections |
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| Relations |
| BioProject |
PRJNA287216 |
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