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Status |
Public on Jun 25, 2015 |
Title |
Human Calu-3 cell transcriptome response to human interferon α [mRNA] |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
The purpose is to obtain samples for mRNA and miRNA analysis with and without interferon α treatment; in parallel, to obtain cell pellets to forward to PNNL for use in top-down proteomics pilot experiments
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Overall design |
Human Calu-3 cells were seeded at 1 x 10^6 cells per well on 6 well plates 3 days before infection. Treatments were initiated by replacing culture medium (containing 10% FBS) with fresh medium containing 500 U/ml of human interferon α (PBL Biosciences). Mock-treated cells were given normal culture medium only. Mock and treated cells were incubated at 37°C until sample collection time points. Infected samples were collected in quadruplet; time-matched mocks were collected in quadruplet in parallel with infected samples. Time points: 6, 12, and 18 h post-infection
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Contributor(s) |
Eisfeld AJ, Stratton K, Waters KM, Kawaoka Y |
Citation(s) |
38565538 |
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Submission date |
Jun 24, 2015 |
Last update date |
Jun 19, 2024 |
Contact name |
Natalie Heller |
E-mail(s) |
natalie.heller@pnnl.gov
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Organization name |
PNNL
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Street address |
902 Battelle Blvd.
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City |
Richland |
ZIP/Postal code |
99354 |
Country |
USA |
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Platforms (1) |
GPL13497 |
Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version) |
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Samples (23)
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This SubSeries is part of SuperSeries: |
GSE65575 |
Modeling Host Responses to Understand Severe Human Virus Infections |
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Relations |
BioProject |
PRJNA287907 |