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Status |
Public on Aug 13, 2015 |
Title |
Mouse lung tissue transcriptome response to influenza H5N1 and mutants [mRNA](Agilent-028005) |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
The purpose is to obtain samples for mRNA, miRNA, proteomics, lipidomics, metabolomics, and histopathology analysis in mouse lung infected with WT A/Vietnam/1203/2004 (H5N1; 'VN1203') and VN1203-NS1trunc124.
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Overall design |
Groups of 22-week-old C57BL/6 mice were infected with WT A/Vietnam/1203/2004 (H5N1; 'VN1203') and VN1203-NS1trunc124 (an NS1 protein with a 90 amino acid truncation at the C-terminus). Infections were done at 10^4 PFU/mouse in 50 μl PBS. Time points were 1, 2, 4, and 7 d.p.i. There were 5 mice for all infection groups and 3 mice for all mock groups. Lung samples were collected for virus load, transcriptional and proteomics analysis. Weight loss and animal survival were also monitored.
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Contributor(s) |
Stratton K, Waters KM, Eisfeld AJ, Kawaoka Y |
Citation(s) |
- Feng S, Heath E, Jefferson B, Joslyn C et al. Hypergraph models of biological networks to identify genes critical to pathogenic viral response. BMC Bioinformatics 2021 May 29;22(1):287. PMID: 34051754
- Eisfeld AJ, Anderson LN, Fan S, Walters KB et al. A compendium of multi-omics data illuminating host responses to lethal human virus infections. Sci Data 2024 Apr 2;11(1):328. PMID: 38565538
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Submission date |
Aug 12, 2015 |
Last update date |
Jun 19, 2024 |
Contact name |
Natalie Heller |
E-mail(s) |
natalie.heller@pnnl.gov
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Organization name |
PNNL
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Street address |
902 Battelle Blvd.
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City |
Richland |
ZIP/Postal code |
99354 |
Country |
USA |
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Platforms (1) |
GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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Samples (37)
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This SubSeries is part of SuperSeries: |
GSE65575 |
Modeling Host Responses to Understand Severe Human Virus Infections |
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Relations |
BioProject |
PRJNA292712 |