|
| Status |
Public on May 15, 2017 |
| Title |
Single-cell transcriptomics uncovers distinct molecular signatures of stem cells in chronic myeloid leukemia |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Single-cell whole transcriptome analysis of chronic myeloid leukemia stem cells, defined as Lin-CD34+CD38-.
|
| |
|
| Overall design |
We developed a method that combines high-sensitivity mutation detection with whole-transcriptome analysis of the same single cell. We applied this technique to analyze more than 2,000 single cells from patients with chronic myeloid leukemia (CML) throughout the disease course, revealing heterogeneity of CML stem cells, including the identification of a subgroup of CML stem cells with a distinct molecular signature that selectively persisted during prolonged therapy. We performed differential gene expression and the gene set enrichment analyses of BCR-ABL+ vs BCR-ABL- single cells from either patients or normal donors, analyzed at different stages of the disease.
|
| |
|
| Contributor(s) |
Thongjuea S, Giustacchini A, Jacobsen SE, Mead A |
| Citation(s) |
28504724 |
| Submission date |
Dec 23, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Claus Nerlov |
| E-mail(s) |
claus.nerlov@imm.ox.ac.uk
|
| Organization name |
Weatheral Institute of Molecular Medicine
|
| Department |
MRC Molecular Haematology Unit
|
| Lab |
Nerlov Laboratory
|
| Street address |
John Radcliffe Hospital, University of Oxford
|
| City |
Oxford |
| ZIP/Postal code |
OX3 9DS |
| Country |
United Kingdom |
| |
|
| Platforms (2) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (2289)
|
|
| Relations |
| BioProject |
PRJNA306878 |
| SRA |
SRP067759 |
Less...
More...