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Status |
Public on Feb 21, 2016 |
Title |
Mouse cerebellum transcriptome response to a wild type West Nile virus (WNV), New York 99 Strain, and mutant WNV-E218A viruses [WCB001_microRNA] |
Organism |
Mus musculus |
Experiment type |
Non-coding RNA profiling by array
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Summary |
The purpose is to obtain samples for mRNA, miRNA, proteomics, lipidomics, metabolomics, and histopathology analysis in mouse cerebellum infected with wild-type West Nile virus (WNV; WNV-NY99 382), and mutant WNV-E218A (WNV-NY99 382 E218A 2 nt).
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Overall design |
Mice were inoculated with WNV intracranially at a dose of 100 FFU. Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Time points: 2 and 4 days post-infection. GSE77161 (WCT001) and this study (WCB001) are 1/4 of cortex and 1/2 of cerebellum from the same mice. Tissues from this experiment are matched to tissues from GSE77161 (WCT001). Infectivity (titer) and pathology are measured from cortex tissue in GSE77161 (WCT001).
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Contributor(s) |
Thackray L, Stratton K, Waters KM, Eisfeld AJ, Kawaoka Y |
Citation(s) |
- Feng S, Heath E, Jefferson B, Joslyn C et al. Hypergraph models of biological networks to identify genes critical to pathogenic viral response. BMC Bioinformatics 2021 May 29;22(1):287. PMID: 34051754
- Eisfeld AJ, Anderson LN, Fan S, Walters KB et al. A compendium of multi-omics data illuminating host responses to lethal human virus infections. Sci Data 2024 Apr 2;11(1):328. PMID: 38565538
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Submission date |
Jan 24, 2016 |
Last update date |
Jun 19, 2024 |
Contact name |
Natalie Heller |
E-mail(s) |
natalie.heller@pnnl.gov
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Organization name |
PNNL
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Street address |
902 Battelle Blvd.
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City |
Richland |
ZIP/Postal code |
99354 |
Country |
USA |
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Platforms (1) |
GPL19970 |
Agilent-046065 Mouse_miRNA_V19.0_Microarray [Probe Name version] |
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Samples (30)
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This SubSeries is part of SuperSeries: |
GSE65575 |
Modeling Host Responses to Understand Severe Human Virus Infections |
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Relations |
BioProject |
PRJNA309681 |