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| Status |
Public on Mar 04, 2016 |
| Title |
Mouse cortex transcriptome response to a wild type West Nile virus (WNV), New York 99 Strain, and mutant WNV-E218A viruses [WCT001_mRNA] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The purpose is to obtain samples for mRNA, miRNA, proteomics, lipidomics, metabolomics, and histopathology analysis in mouse cortex infected with wild-type West Nile virus (WNV; WNV-NY99 382), and mutant WNV-E218A (WNV-NY99 382 E218A 2 nt).
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| Overall design |
Mice were inoculated with WNV intracranially at a dose of 100 FFU. Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Time points: 1, 2, 4, and 6 days post-infection. This study (WCT001) and GSE77192 (WCB001) are 1/4 of cortex and 1/2 of cerebellum from the same mice. Tissues from this experiment are matched to tissues from GSE77192 (WCB001). Infectivity (titer) and pathology are measured from the tissue in WCT001 (i.e., this experiment).
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| Contributor(s) |
Thackray L, Stratton K, Waters KM, Eisfeld AJ, Kawaoka Y |
| Citation(s) |
34051754 |
| Submission date |
Jan 25, 2016 |
| Last update date |
Aug 24, 2021 |
| Contact name |
Natalie Heller |
| E-mail(s) |
natalie.heller@pnnl.gov
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| Organization name |
PNNL
|
| Street address |
902 Battelle Blvd.
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| City |
Richland |
| ZIP/Postal code |
99354 |
| Country |
USA |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (60)
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| This SubSeries is part of SuperSeries: |
| GSE65575 |
Modeling Host Responses to Understand Severe Human Virus Infections |
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| Relations |
| BioProject |
PRJNA309682 |
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