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| Status |
Public on May 07, 2016 |
| Title |
Human liver Huh cell transcriptome (mRNA) response to a Wild-type ΔVP30 Ebola virus, Δmucin, and ssGP mutants |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The purpose is to obtain samples for mRNA analysis in human liver Huh cells using the following viruses obtained by reverse genetics: Zaire Ebola virus wild-type (WT) in the ΔVP30 background (wild), ΔsGP virus (eliminates expression of soluble glycoprotein [GP]) (ssGP), and Δmucin virus (encodes a GP lacking the mucin domain) (mucin).
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| Overall design |
Human liver Huh cells were infected with a Wild-type ΔVP30 Ebola virus, Δmucin, and ssGP mutants. The Huh7.0 VP30 cells were on split 5 for this experiment. Infected samples were collected in quintuplet; time-matched mocks were collected in quintuplet in parallel with infected samples. Two time courses were performed to generate samples for this experiment (the second set was started within 24 h of the first set). The time courses were 00, 8, and 18 hours, and 0, 24, and 48 hours.
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| Contributor(s) |
Halfmann P, Thompson A, Waters KM, Eisfeld AJ, Kawaoka Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 08, 2016 |
| Last update date |
Jan 09, 2018 |
| Contact name |
Natalie Heller |
| E-mail(s) |
natalie.heller@pnnl.gov
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| Organization name |
PNNL
|
| Street address |
902 Battelle Blvd.
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| City |
Richland |
| ZIP/Postal code |
99354 |
| Country |
USA |
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| Platforms (1) |
| GPL13497 |
Agilent-026652 Whole Human Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (119)
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| This SubSeries is part of SuperSeries: |
| GSE65575 |
Modeling Host Responses to Understand Severe Human Virus Infections |
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| Relations |
| BioProject |
PRJNA317724 |
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