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GEO help: Mouse over screen elements for information. |
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| Status |
Public on May 23, 2022 |
| Title |
Molecular signature of anastasis for reversal of apoptosis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We recently discovered an unexpected reversibility of execution-stage apoptosis in vitro and in vivo, and coined the term anastasis (Greek for “rising to life”) for this cell recovery phenomenon. Promoting anastasis could in principle preserve injured cells that are difficult to replace such as cardiomyocytes and neurons. Conversely, arresting anastasis in dying cancer cells after cancer therapies could improve treatment efficacy. To harness the discovery of anastasis to develop revolutionary new therapies, it is essential to identify the key regulators of anastasis – the therapeutic targets. Therefore, we performed microarray analysis to study the molecular mechanism of anastasis using reversal of ethanol-induced apoptosis in mouse primary liver cells as a model. Our data reveal active transcription involved in multiple pathways during anastasis, including early activation of pro-survival genes, cell cycle arrest, anti-p53-mediated DNA damage response, and at delayed times, stress-inducible responses such as cell migration. Here, we present a dataset containing the time-course gene expression profiles during apoptosis reversal, which has implications for the physiological, pathological, and therapeutic implications of anastasis.
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| Overall design |
Mouse primary liver cells were treated with 4.5% ethanol for 5 hours (0 hour timepoint) and then washed and cultured in fresh medium for 3, 6, 24, and 48 hours. Three biological replicates were analyzed for each of 6 timepoints: pre-ethanol treament (Ctrl), 0 hours (R0), 3 hours (R3), 6 hours (R6), 24 hours (R24), and 48 hours (R48).
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| Contributor(s) |
Tang H, Tang H, Fung M |
| Citation(s) |
35851273 |
| Submission date |
Dec 06, 2016 |
| Last update date |
Aug 23, 2022 |
| Contact name |
Ho Lam Tang |
| E-mail(s) |
htang10@jhmi.edu
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| Organization name |
The Johns Hopkins University School of Medicine
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| Department |
Neurosurgery
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| Lab |
E5140 Bloomberg School of Public Health Building
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| Street address |
615 N. Wolfe Street
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (18)
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| GSM2418377 |
Ctrl (Untreated mouse primary liver cells) Replicate 1 |
| GSM2418378 |
Ctrl (Untreated mouse primary liver cells) Replicate 2 |
| GSM2418379 |
Ctrl (Untreated mouse primary liver cells) Replicate 3 |
| GSM2418380 |
R0 (Cells were treated with 4.5% ethanol in culture medium for 5 hours. Replicate 1 |
| GSM2418381 |
R0 (Cells were treated with 4.5% ethanol in culture medium for 5 hours) Replicate 2 |
| GSM2418382 |
R0 (Cells were treated with 4.5% ethanol in culture medium for 5 hours) Replicate 3 |
| GSM2418383 |
R3 (Treated cells were washed with fresh culture medium, and then incubated for 3 hours) Replicate 1 |
| GSM2418384 |
R3 (Treated cells were washed with fresh culture medium, and then incubated for 3 hours) Replicate 2 |
| GSM2418385 |
R3 (Treated cells were washed with fresh culture medium, and then incubated for 3 hours) Replicate 3 |
| GSM2418386 |
R6 (Treated cells were washed with fresh culture medium, and then incubated for 6 hours) Replicate 1 |
| GSM2418387 |
R6 (Treated cells were washed with fresh culture medium, and then incubated for 6 hours) Replicate 2 |
| GSM2418388 |
R6 (Treated cells were washed with fresh culture medium, and then incubated for 6 hours) Replicate 3 |
| GSM2418389 |
R24 (Treated cells were washed with fresh culture medium, and then incubated for 24 hours) Replicate 1 |
| GSM2418390 |
R24 (Treated cells were washed with fresh culture medium, and then incubated for 24 hours) Replicate 2 |
| GSM2418391 |
R24 (Treated cells were washed with fresh culture medium, and then incubated for 24 hours) Replicate 3 |
| GSM2418392 |
R48 (Treated cells were washed with fresh culture medium, and then incubated for 48 hours) Replicate 1 |
| GSM2418393 |
R48 (Treated cells were washed with fresh culture medium, and then incubated for 48 hours) Replicate 2 |
| GSM2418394 |
R48 (Treated cells were washed with fresh culture medium, and then incubated for 48 hours) Replicate 3 |
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| Relations |
| BioProject |
PRJNA356448 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE90959_RAW.tar |
15.8 Mb |
(http)(custom) |
TAR |
| GSE90959_non-normalized.txt.gz |
9.6 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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