|
| Status |
Public on May 23, 2022 |
| Title |
R0 (Cells were treated with 4.5% ethanol in culture medium for 5 hours) Replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
mouse primary liver cells
|
| Organism |
Mus musculus |
| Characteristics |
treatment: 4.5% EtOH, 5Hr
|
| Treatment protocol |
Mouse primary liver cells were treated with 4.5% ethanol for 5 hours (R0) and then washed and cultured in fresh DMEM F12 10% FBS medium for 3 (R3), 6 (R6), 24 (R24), and 48 (R48) hours.
|
| Growth protocol |
Primary liver cells were isolated from BALB/c mice by collagenase B according to the manufacturer’s instructions (Worthington Biochemical, Lakewood, NJ) and cultured as described (PMID: 22535522, DOI: 10.1091/mbc.E11-11-0926)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA of all samples was extracted with Trizol reagent. Total RNA was isolated and purified by RNeasy Mini Kit (Qiagen, Cologne, Germany).
|
| Label |
Cy3
|
| Label protocol |
The RNA was labelled with the SABiosciences C-03 RT² First Strand Kit (SABiosciences - QIAGEN, Frederick, MD) standard protocol
|
| |
|
| Hybridization protocol |
Labeled cRNA was combined with hybridization buffer and hybridized to the array at 58°C for 16-20 hours with rocking
|
| Scan protocol |
Arrays were scanned with the Illumina iScan BeadArray scanner standard protocol
|
| Data processing |
Data were extracted with the Illumina BeadStudio with Gene Expression module, average normalization, to create an output file. These values were imported into Spotfire DecisionSite v9.1.2 and log2 transformed.
|
| |
|
| Submission date |
Dec 06, 2016 |
| Last update date |
May 23, 2022 |
| Contact name |
Ho Lam Tang |
| E-mail(s) |
htang10@jhmi.edu
|
| Organization name |
The Johns Hopkins University School of Medicine
|
| Department |
Neurosurgery
|
| Lab |
E5140 Bloomberg School of Public Health Building
|
| Street address |
615 N. Wolfe Street
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE90959 |
Molecular signature of anastasis for reversal of apoptosis |
|
