|
| Status |
Public on Dec 31, 2019 |
| Title |
primary bovine trophoblast giant cells from cow 2 |
| Sample type |
RNA |
| |
|
| Source name |
primary bovine trophoblast giant cells
|
| Organism |
Bos taurus |
| Characteristics |
cell type: primary bovine trophoblast giant cells
|
| Treatment protocol |
Primary UTC and TGC isolates were analyzed without further treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared using the NucleoSpin RNA Kit including removal of genomic DNA with RNAse free rDNAse (Macherey-Nagel).
|
| Label |
Biotin
|
| Label protocol |
RNA from each cell sample was used to generate single strand DNA (Ambion WT Expression Kit). Fragmentation and labeling was performed with the Affymetrix Gene Chip WT Terminal Labeling and Hybridization Kit
|
| |
|
| Hybridization protocol |
The RNA was hybridized overnight (45°C) in the GeneChipR Hybridization Oven (Affymetrix) to the Affymetrix Bovine Gene 1.0 ST arrays. Washing and staining were performed according to the Affymetrix standard protocol.
|
| Scan protocol |
The scanning of the microarray was done with the GeneChip Scanner 3000 7G (Affymetrix) and image processing was done with the software AGCC (Affymetrix).
|
| Data processing |
Raw data were processed with the Expression Console (V1.4.1.46; Affymetrix), where normalization, background reduction and a gene-level summary was done using the RMA method (Robust Multichip Average).
|
| |
|
| Submission date |
Nov 13, 2018 |
| Last update date |
Jan 24, 2020 |
| Contact name |
Rainer Fuerbass |
| E-mail(s) |
fuerbass@fbn-dummerstorf.de
|
| Organization name |
Leibniz Institute for Farm Animal Biology
|
| Street address |
Wilhelm-Stahl-Allee
|
| City |
Dummerstorf |
| ZIP/Postal code |
18196 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16500 |
| Series (1) |
| GSE122474 |
Transcriptome analysis of bovine uninucleated trophoblast cells (UTC) and Trophoblast Giant Cells (TGC) |
|
