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Sample GSM5201869 Query DataSets for GSM5201869
Status Public on Jun 14, 2021
Title DCIS_normal_rep1
Sample type RNA
 
Source name Non-tumorous region, MI-FTC, FFPE
Organism Homo sapiens
Characteristics individual: DCIS patient 1
tissue: Mammary gland
tissue region: normal
gender: female
age: 40
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using RNAiso reagent (Takara-bio, Kusatsu, Japan).
Label biotin
Label protocol Biotinylated cRNA were prepared usibg the GeneChip® WT Pico Reagent Kit from 100 ng total RNA (GeneChip® WT Pico Reagent Kit User Manual, Affymetrix).
 
Hybridization protocol Fifteen ug of cRNA were subjected to fragmentation using the 3' fragmentation buffer hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the GeneChip® Scanner 3000 7G.
Description RNA from biopsy specimens
Data processing The data were analyzed with Affymetrix Transcriptome Analysis Console® using RMA as a summerization algorithm.
 
Submission date Mar 23, 2021
Last update date Jun 16, 2021
Contact name OSAMU ISHIBASHI
E-mail(s) ishibashi@biochem.osakafu-u.ac.jp
Phone +81-72-254-9474
Organization name Osaka Prefecture University
Department Grad Sch Life & Environmental Sciences
Lab Biological Macromolecules
Street address 1-1 Gakuen-cho, Naka-ku
City Sakai
ZIP/Postal code 599-8531
Country Japan
 
Platform ID GPL23126
Series (1)
GSE169393 Array-based genome-wide transcriptome analysis of Ductal carcinoma in situ (DCIS)

Data table header descriptions
ID_REF
VALUE Bi-weight Avg Signal (log2)

Data table
ID_REF VALUE
TC0100006432.hg.1 3
TC0100006433.hg.1 4.67
TC0100006434.hg.1 5
TC0100006435.hg.1 3
TC0100006436.hg.1 4.58
TC0100006437.hg.1 3.73
TC0100006438.hg.1 9.03
TC0100006439.hg.1 8.4
TC0100006440.hg.1 5.41
TC0100006441.hg.1 4.85
TC0100006442.hg.1 3.41
TC0100006443.hg.1 5.29
TC0100006444.hg.1 5.39
TC0100006445.hg.1 4.14
TC0100006446.hg.1 6.18
TC0100006447.hg.1 18.35
TC0100006448.hg.1 6.22
TC0100006449.hg.1 3.26
TC0100006450.hg.1 3.97
TC0100006451.hg.1 5.19

Total number of rows: 135750

Table truncated, full table size 3051 Kbytes.




Supplementary file Size Download File type/resource
GSM5201869_EA1777_02.CEL.gz 22.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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