1 match for GPM32310002988,
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| aaa
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| GO | path | snaps | mh | ζ |
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BRENDA cell culture: none BRENDA tissue: none CELL cell type: none GO subcellular: none email: gert.lubec@meduniwien.ac.at institution: Department of Pediatrics, Medical University of Vienna, Vienna, Austria name: Heo S, et al. project: Gel-based mass spectrometric analysis of hippocampal transmembrane proteins using high resolution LTQ Orbitrap Velos Pro. project comment: Data from ProteomeXchange, PXD000311. Enzyme: trypsin, file: F017572.dat-ACA-SDS_T3.mzml. Published as part of Proteomics. 2014 Jul 10 (PubMed ). From the Abstract: ... Proteins were extracted from pooled whole mouse brains, enriched membrane fractions were prepared using either two commercially available kits or 6-aminocaproic acid under denaturing or native conditions followed by gel-based proteomic approaches using blue native (BN-) and SDS-PAGE with subsequent in-gel digestion with several proteases, chymotrypsin, trypsin followed by nano-LC-ESI-MS/MS analysis on LTQ Orbitrap Velos Pro ... |
Notes:
- Each of the diagrams indicates the patterns of peptides observed for proteins in a particular set of protein observations, identified
by its GPM accession number. These patterns of
experimentally determined peptides are commonly referred to as "protein sequence coverage" displays.
- The diagram elements are as follows:
- black line: indicates the full length of the protein (all sequences are the same length in this display)
- red bars: indicate observed peptides (the darker the red, the more confident the observations); and
- green bars: indicate regions of the protein that may be difficult to observe, using standard proteomics techniques;
- [X/Y]: the rank of a particular observation, with respect to all of the data in GPMDB.
e.g., [3/10] means that this
would be the 3rd best observation of this protein, out of 10 total observations.
- Clicking on the links to the left of the diagrams take you to a full display of the peptides and
protein sequence information for that protein.
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