Entry Name Mitophagy - yeast - Saccharomyces cerevisiae (budding yeast)
Description Mitophagy, which refers to the selective elimination of impaired or excessive mitochondria, is considered to be the main mechanism for mitochondria quality and quantity control. In yeast, oxidative stress or inhibition of TOR induces expression of Agt32, the indispensable mitochondrial outer membrane receptor. Direct phosphorylation of Atg32 by CK2 triggers mitophagy, and two mitogen-activated protein kinase (MAPK) signal transduction pathways are also important in this process. Atg11 is an adaptor protein for selective autophagy, and recruits the cargo to the phagophore assembly site (PAS), where the autophagosome is generated. Then Atg32-Atg8 interaction enhances the formation of the autophagosome surrounding the mitochondria, which finally fuses with vacuoles for degradation. Atg32-Atg11 interaction may also be regulated by Yme1-mediated processing of Atg32, as well as by mitochondrial fission machinery. Some factors have been suggested to positively or negatively regulate the mitophagy via different mechanisms, including Atg1, Atg33, Mss4, Fmc1, Mdm38, Mip1, and Ubp3-Bre5 deubiquitination complex.
Class Cellular Processes; Transport and catabolismBRITE hierarchy
Pathway map Other DBs Organism Saccharomyces cerevisiae (budding yeast) [GN:
sce ]
Gene YDR207C UME6; DNA-binding transcriptional regulator UME6 [KO:K09243 ]
YGL019W CKB1; casein kinase 2 regulatory subunit CKB1 [KO:K03115 ]
YLR006C SSK1; mitogen-activated protein kinase kinase kinase SSK1 [KO:K11233 ]
YOR039W CKB2; casein kinase 2 regulatory subunit CKB2 [KO:K03115 ]
Compound D00753 Sirolimus (JAN/USAN/INN)
Reference Authors Kanki T, Furukawa K, Yamashita S
Title Mitophagy in yeast: Molecular mechanisms and physiological role.
Journal Reference Authors Muller M, Lu K, Reichert AS
Title Mitophagy and mitochondrial dynamics in Saccharomyces cerevisiae.
Journal Reference Authors Okamoto K, Kondo-Okamoto N, Ohsumi Y
Title Mitochondria-anchored receptor Atg32 mediates degradation of mitochondria via selective autophagy.
Journal Reference Authors Kanki T, Wang K, Cao Y, Baba M, Klionsky DJ
Title Atg32 is a mitochondrial protein that confers selectivity during mitophagy.
Journal Reference Authors Mao K, Wang K, Zhao M, Xu T, Klionsky DJ
Title Two MAPK-signaling pathways are required for mitophagy in Saccharomyces cerevisiae.
Journal Reference Authors Aoki Y, Kanki T, Hirota Y, Kurihara Y, Saigusa T, Uchiumi T, Kang D
Title Phosphorylation of Serine 114 on Atg32 mediates mitophagy.
Journal Reference Authors Kanki T, Kurihara Y, Jin X, Goda T, Ono Y, Aihara M, Hirota Y, Saigusa T, Aoki Y, Uchiumi T, Kang D
Title Casein kinase 2 is essential for mitophagy.
Journal Reference Authors Kondo-Okamoto N, Noda NN, Suzuki SW, Nakatogawa H, Takahashi I, Matsunami M, Hashimoto A, Inagaki F, Ohsumi Y, Okamoto K
Title Autophagy-related protein 32 acts as autophagic degron and directly initiates mitophagy.
Journal Reference Authors Mao K, Wang K, Liu X, Klionsky DJ
Title The scaffold protein Atg11 recruits fission machinery to drive selective mitochondria degradation by autophagy.
Journal Reference Authors Wang K, Jin M, Liu X, Klionsky DJ
Title Proteolytic processing of Atg32 by the mitochondrial i-AAA protease Yme1 regulates mitophagy.
Journal Reference Authors Tal R, Winter G, Ecker N, Klionsky DJ, Abeliovich H
Title Aup1p, a yeast mitochondrial protein phosphatase homolog, is required for efficient stationary phase mitophagy and cell survival.
Journal Reference Authors Kanki T, Wang K, Baba M, Bartholomew CR, Lynch-Day MA, Du Z, Geng J, Mao K, Yang Z, Yen WL, Klionsky DJ
Title A genomic screen for yeast mutants defective in selective mitochondria autophagy.
Journal Reference Authors Wang K, Yang Z, Liu X, Mao K, Nair U, Klionsky DJ
Title Phosphatidylinositol 4-kinases are required for autophagic membrane trafficking.
Journal Reference Authors Priault M, Salin B, Schaeffer J, Vallette FM, di Rago JP, Martinou JC
Title Impairing the bioenergetic status and the biogenesis of mitochondria triggers mitophagy in yeast.
Journal Reference Authors Nowikovsky K, Reipert S, Devenish RJ, Schweyen RJ
Title Mdm38 protein depletion causes loss of mitochondrial K+/H+ exchange activity, osmotic swelling and mitophagy.
Journal Reference Authors Zhang Y, Qi H, Taylor R, Xu W, Liu LF, Jin S
Title The role of autophagy in mitochondria maintenance: characterization of mitochondrial functions in autophagy-deficient S. cerevisiae strains.
Journal Reference Authors Muller M, Kotter P, Behrendt C, Walter E, Scheckhuber CQ, Entian KD, Reichert AS
Title Synthetic quantitative array technology identifies the Ubp3-Bre5 deubiquitinase complex as a negative regulator of mitophagy.
Journal Related KO pathway 
