Photoaffinity probe-based protein labeling of microbial isolate Acinetobacter pittii SO1 (NCBITAXON:48296), isolated from the sorghum rhizosphere that can utilize sorgoleone as a sole carbon source in order to identify proteins that bind sorgoleone. Photoaffinity labeling of A. pittii lysates using the SoDA-PAL probe approach may be extended in the future to proteomic profiling of complex rhizosphere microbiomes to discover genes that can be leveraged to promote beneficial plant-microbe interactions (grown on both acetate and purified sorgoleone). Eluted peptides from the C18 column were analyzed using a Q-Exactive Plus Orbitrap for high resolution MS and high-energy collision-induced dissociation tandem MS by electrospray ionization for subsequent quantitative proteomic analysis.
[doi:10.25345/C5P55DS70]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: sorghum rhizosphere ; microbial proteomics ; LC-MS ; peptide quantitation ; PerCon SFA ; SoDA-PAL probe proteomics ; sorgoleone catabolism
Principal Investigators: (in alphabetical order) |
Robert G. Egbert, Pacific Northwest National Laboratory, United States |
Submitting User: | alchemistmatt |
Van Fossen EM, Kroll JO, Anderson LN, McNaughton AD, Herrera D, Oda Y, Wilson AJ, Nelson WC, Kumar N, Frank AR, Elmore JR, Handakumbura P, Lin VS, Egbert RG.
Profiling sorghum-microbe interactions with a specialized photoaffinity probe identifies key sorgoleone binders in Acinetobacter pittii.
Appl Environ Microbiol. Epub 2024 Sep 9.
Jared O. Kroll, Elise M. Van Fossen, Lindsey N. Anderson, Andrew D. McNaughton, Daisy Herrera, Yasuhiro Oda, Andrew J. Wilson, William C. Nelson, Neeraj Kumar, Andrew R. Frank, Joshua R. Elmore, Pubudu Handakumbura, Vivian S. Lin, Robert G. Egbert.
Profiling sorghum-microbe interactions with a specialized photoaffinity probe identifies key sorgoleone binders in Acinetobacter pittii.
bioRxiv 2023.05.31.542302; doi: https://doi.org/10.1101/2023.05.31.542302.
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