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SRX17620955: insect sample
1 ILLUMINA (Illumina NovaSeq 6000) run: 86.4M spots, 25.9G bases, 14.3Gb downloads

Design: To anchor hybrid scaffolds onto the chromosome, genomic DNA was extracted for the Hi-C library from XXX. Then, we constructed the Hi-C library and obtained sequencing data via the Illumina Novaseq/MGI-2000 platform. In brief, freshly harvested leaves were cut into 2 cm pieces and vacuum infiltrated in nuclei isolation buffer supplemented with 2% formaldehyde. Crosslinking was stopped by adding glycine and additional vacuum infiltration. Fixed tissue was then grounded to powder before re-suspending in nuclei isolation buffer to obtain a suspension of nuclei. The purified nuclei were digested with 100 units of DpnII and marked by incubating with biotin-14-dATP. Biotin-14-dATP from non-ligated DNA ends was removed owing to the exonuclease activity of T4 DNA polymerase. The ligated DNA was sheared into 300600 bp fragments, and then was blunt-end repaired and Atailed, followed by purification through biotin-streptavidin-mediated pull down. Finally, the Hi-C libraries were quantified and sequenced using the Illumina Novaseq/MGI-2000 platform.
Submitted by: Zhejiang University
Study: Chromosome-level genome assembly of Chouioia cunea Yang, the parasitic wasp of the fall webworm
show Abstracthide Abstract
Chouioia cunea Yang 1989 is a parasitic wasp and natural enemy of several lepidopteran pests during their pupal stage, this wasp has successfully used to control pests such as the fall webworm. Here we reported the first chromosome-level genome of Eulophidae. We applied short, long, chromatin-linked sequencing reads and transcriptomic data to assemble the chromosome-level genome of C. cunea. The version of the genome assembly of C. cunea was highly contiguous with a total assembly length of 171.99 Mb contained in 11 contigs with a GC content of 36.89%, the scaffold/contig N50 length was 31.70/26.52Mb. The Benchmarking Universal Single-Copy Orthologs completeness score is 98.70% at the protein level, including 1,318 single-copy genes and 32 duplicated genes. Repeat sequences accounted for 15.15% of the whole-genome assembly. A total of 12,258 protein-coding genes were annotated in this genome. We also predicted 324 noncoding RNAs including 84 micro-RNAs, 69 ribosomal RNAs, 28 small nuclear RNAs, 205 transfer RNAs, and 44 others. This high-quality genome of C. cunea is an important step toward bettering our knowledge of the Eulophidae, it will serve as a valuable resource for phylogenetic relationships and a better understanding of the evolution of Chalcidoidea.
Sample: Chouioia cunea
SAMN30915915 • SRS15156678 • All experiments • All runs
Organism: Chouioia cunea
Library:
Name: ZJUG0153-Hic1
Instrument: Illumina NovaSeq 6000
Strategy: Hi-C
Source: GENOMIC
Selection: PCR
Layout: PAIRED
Runs: 1 run, 86.4M spots, 25.9G bases, 14.3Gb
Run# of Spots# of BasesSizePublished
SRR2162008386,431,32225.9G14.3Gb2022-09-19

ID:
24513488

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