SRX23859327: Mal_Hi-C
1 DNBSEQ (DNBSEQ-T7) run: 484.6M spots, 145.4G bases, 77.3Gb downloads
Design: The tissue was vacuum infiltrated in nuclei isolation buffer supplemented with 2% formaldehyde. Adding glycine and additional vacuum infiltration to stop the crosslinking. Fixed tissue was grounded to powder and re-suspended in nuclei isolation buffer. The purified nuclei were digested with 100units of DpnII and marked by incubating with biotin-14-dATP. Removed biotin-14-dATP from non-ligated DNA ends using T4 DNA polymerase. The ligated DNA was sheared into 300-600 bp fragments, and then was blunt-end repaired and A-tailed, followed by purification through biotin-streptavidin-mediated pull down.
Submitted by: Hebei University
Study:
Genome of Monochamus alternatusshow Abstracthide AbstractWhole-genome sequencing data of forest pest Monochamus alternatus.
Library:
Name: L3
Instrument: DNBSEQ-T7
Strategy: Hi-C
Source: GENOMIC
Selection: other
Layout: PAIRED
Runs:
1 run, 484.6M spots, 145.4G bases, 77.3Gb