Illumina sequencing of Myxobolus honghuensis spores RNA - SRA

SRX13090597: Illumina sequencing of Myxobolus honghuensis spores RNA
1 ILLUMINA (Illumina HiSeq 2500) run: 24.9M spots, 6.2G bases, 2.8Gb downloads

Design: Myxobolus honghuensis was collected from infected allogynogenetic gibel carp Carassius auratus gibelio in Zoumaling Farm, Hubei Province, China on July 29th, 2015. We have 3 RNA candidates (from three different fishes) for Illumina transcriptome sequencing. Before RNA extraction, we checked the three samples and found immature plasmodia that contained spores of different stages. Then we extracted the RNA separately, tested the RNA quality, and combined the RNA. RNA was isolated with TRIzol 550 (Invitrogen). The purity and integrity of RNA were assessed using NanoPhotometer? spectrophotometer (IMPLEN, CA, USA) and RNA Nano 6000 Assay Kit of the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA) respectively. Only one library was constructed from the combined RNA and sent for RNA sequencing. Libraries were prepared using NEBNext? Ultra RNA kit (New England Biolab (NEB), Ipswich, MA) following manufacturer's recommendations and sequenced as 2 125 paired-end (PE) runs with Illumina HiSeq 2500.

Submitted by: Huazhong Agricultural Univeristy

Study: Myxobolus honghuensis transcriptome sequencing for genomic analysis

PRJNA779260 • SRP345383 • All experiments • All runs

show Abstracthide Abstract

Parasite evolution has been conceptualized as a process of genetic loss and simplification. Contrary to this model, there is evidence of expansion and conservation of gene families related to essential functions of parasitism in some parasite genomes, reminiscent of widespread mosaic evolution, where subregions of a genome have different rates of evolutionary change. We found evidence of mosaic genome evolution in the cnidarian Myxobolus honghuensis, a myxozoan parasite of fish, with extremely simple morphology.

Sample: Myxozoan species Myxobolus honghuensis sample from Wuhan, China. Used for assembling a transcriptome.

SAMN23005370 • SRS11026986 • All experiments • All runs

Organism: Myxobolus honghuensis

Library:

Name: MH-illumina-RNA-2015

Instrument: Illumina HiSeq 2500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: RANDOM

Layout: PAIRED

Runs: 1 run, 24.9M spots, 6.2G bases, 2.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR16897929	24,920,204	6.2G	2.8Gb	2022-02-02

ID:: 17801012

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