RNA-Seq of human Vdelta1 T cells from peripheral blood. Donor 2, delt... - SRA

SRX4717060: RNA-Seq of human Vdelta1 T cells from peripheral blood. Donor 2, delta chain
1 ILLUMINA (Illumina MiSeq) run: 149,704 spots, 42.4M bases, 27.4Mb downloads

Design: Flow cytometric sorted cells were subjected to RNA extraction using the RNAeasy mini kit (Qiagen). Next, 12l mRNA of each sample was reverse transcribed with the Superscript III enzyme kit (Invitrogen). CDR3 TRG and TRD sequencing amplicons were generated as described previously (Ravens et al. Nat Immunology 2017), while using 25 30 PCR cycles. According to Illumina guidelines 96-samples were labeled with Nextera XT indices and subjected to Illumina MiSeq analysis (500 cycle; paired-end sequencing), while 20% PhIX was added as a control.

Submitted by: Instituto de Medicina Molecular Joao Lobo Antunes

Study: HTS data of human gamma and delta repertoires from peripheral blood, DOT and naive vs differentiated V delta 1 T cells

PRJNA491919 • SRP162140 • All experiments • All runs

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This study aims at characterizing the gamma and delta repertoires of human V delta 1 T cells of different origins:- Sorted Vdelta1 T cells from peripheral blood;- Sorted Vdelta1 T cells from DOT cells (Almeida, Clin Cancer Res, 2016);- Sorted naive CD27+ naive and CD27- differentiated Vdelta1 Tcells from peripheral blood.

Sample:

SAMN10092789 • SRS3802701 • All experiments • All runs

Organism: Homo sapiens

Library:

Name: HD2_PB_delta

Instrument: Illumina MiSeq

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 149,704 spots, 42.4M bases, 27.4Mb

Run	# of Spots	# of Bases	Size	Published
SRR7878385	149,704	42.4M	27.4Mb	2019-03-14

ID:: 6384890

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