MCM5 MCM DNA helicase complex subunit MCM5 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: MCM5
Gene description: MCM DNA helicase complex subunit MCM5
Primary source: SGD:S000004264
Locus tag: YLR274W
See related: AllianceGenome:SGD:S000004264
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: BOB1; CDC46
Summary: Enables DNA replication origin binding activity and chromatin binding activity. Contributes to single-stranded DNA binding activity and single-stranded DNA helicase activity. Involved in several processes, including cellular macromolecule biosynthetic process; double-strand break repair via break-induced replication; and subtelomeric heterochromatin assembly. Located in chromosome, telomeric region; cytoplasm; and nucleus. Part of MCM complex and nuclear lumen. Used to study Meier-Gorlin syndrome. Human ortholog(s) of this gene implicated in Meier-Gorlin syndrome 8. Orthologous to human MCM5 (minichromosome maintenance complex component 5). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (691555..693882)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The Mcm ATPase active sites that flank Mcm2/5 (i.e. Mcm5/3 and 6/2) modulate the biochemical activities of the putative Mcm2/5 gate.
Title: The Saccharomyces cerevisiae Mcm6/2 and Mcm5/3 ATPase active sites contribute to the function of the putative Mcm2-7 'gate'.
DNA runs through a central channel in the double hexamer, and, once loaded, Mcm2-7 can slide passively along double-stranded DNA.
Title: Concerted loading of Mcm2-7 double hexamers around DNA during DNA replication origin licensing.
Conclusions predict a structural discontinuity between Mcm2 and Mcm5 and demonstrate that in contrast to other hexameric helicases, the six Mcm2-7 active sites are functionally distinct.
Title: Subunit organization of Mcm2-7 and the unequal role of active sites in ATP hydrolysis and viability.
Mcm5 protein has a positive role in origin binding, which requires coordination by all six Mcm2-7 subunits in the hexamer.
Title: Functional conservation of beta-hairpin DNA binding domains in the Mcm protein of Methanobacterium thermoautotrophicum and the Mcm5 protein of Saccharomyces cerevisiae.
A domain-push model is proposed to explain the bypass activity of mutant proline62leucine BOB1, which has a major effect on helicase activity, but no significant impact on DNA binding or ATPase of Methanobacterium thermoautotrophicum MCM gene.
Title: Biochemical activities of the BOB1 mutant in Methanobacterium thermoautotrophicum MCM.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
contributes_to 3'-5' DNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA replication origin binding	IDA Inferred from Direct Assay more info	PubMed
enables DNA replication origin binding	IEA Inferred from Electronic Annotation more info
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables helicase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
contributes_to single-stranded DNA helicase activity	IBA Inferred from Biological aspect of Ancestor more info
contributes_to single-stranded DNA helicase activity	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA duplex unwinding	IEA Inferred from Electronic Annotation more info
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA replication initiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA replication initiation	IEA Inferred from Electronic Annotation more info
involved_in DNA replication initiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA unwinding involved in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in cell cycle	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via break-induced replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair via break-induced replication	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via break-induced replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pre-replicative complex assembly involved in nuclear cell cycle DNA replication	IDA Inferred from Direct Assay more info	PubMed
involved_in pre-replicative complex assembly involved in nuclear cell cycle DNA replication	IEA Inferred from Electronic Annotation more info
involved_in premeiotic DNA replication	IEA Inferred from Electronic Annotation more info
involved_in regulation of DNA-templated DNA replication initiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in subtelomeric heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transcription elongation by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of CMG complex	IDA Inferred from Direct Assay more info	PubMed
part_of CMG complex	IEA Inferred from Electronic Annotation more info
part_of DNA replication preinitiation complex	IPI Inferred from Physical Interaction more info	PubMed
part_of MCM complex	IBA Inferred from Biological aspect of Ancestor more info
part_of MCM complex	IDA Inferred from Direct Assay more info	PubMed
part_of MCM complex	IEA Inferred from Electronic Annotation more info
located_in chromosome, telomeric region	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
part_of nuclear pre-replicative complex	IDA Inferred from Direct Assay more info	PubMed
part_of nuclear pre-replicative complex	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of replication fork protection complex	IDA Inferred from Direct Assay more info	PubMed
part_of replication fork protection complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: MCM DNA helicase complex subunit MCM5

NP_013376.1

EC 3.6.4.12

Component of the Mcm2-7 hexameric helicase complex; MCM complex is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

691555..693882

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182161.1 → NP_013376.1 TPA: MCM DNA helicase complex subunit MCM5 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013376.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VYS1, P29496

UniProtKB/TrEMBL

A0A6A5PUY8, A7A1F8, B3RHF6, B5VNI6, C7GVT5, C8ZDN8, G2WJA0, N1NYH9

Conserved Domains (2) summary

smart00350
Location:144 → 689

MCM; minichromosome maintenance proteins

pfam14551
Location:29 → 101

MCM_N; MCM N-terminal domain