ERG9 bifunctional farnesyl-diphosphate farnesyltransferase/squalene synthase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: ERG9
Gene description: bifunctional farnesyl-diphosphate farnesyltransferase/squalene synthase
Primary source: SGD:S000001233
Locus tag: YHR190W
See related: AllianceGenome:SGD:S000001233
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables farnesyl-diphosphate farnesyltransferase activity and squalene synthase activity. Involved in ergosterol biosynthetic process. Is integral component of membrane. Orthologous to human FDFT1 (farnesyl-diphosphate farnesyltransferase 1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See ERG9 in Genome Data Viewer

Location:: chromosome: VIII

Exon count:: 1

Sequence:: Chromosome: VIII; NC_001140.6 (484845..486179)

Chromosome VIII - NC_001140.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001140.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Restoration of the complete complementation phenotype was mapped to a 26-amino acid hinge region linking the catalytic and membrane-spanning domains specific to fungal squalene synthases.
Title: Mapping a kingdom-specific functional domain of squalene synthase.
Data show that engineered strains improved amorpha-4,11-diene by 2~5-fold over the reference strain with squalene synthase ERG9 under its native promoter.
Title: Dynamic control of ERG9 expression for improved amorpha-4,11-diene production in Saccharomyces cerevisiae.
The product distribution in the strains indicated that the energy barrier could be the reason that different metal ion-assisted electrophilic attacks initiated carbocation formation.
Title: ERG9 and COQ1 disruption reveals isoprenoids biosynthesis is closely related to mitochondrial function in Saccharomyces cerevisiae.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables farnesyl-diphosphate farnesyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables farnesyl-diphosphate farnesyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables farnesyl-diphosphate farnesyltransferase activity	IEA Inferred from Electronic Annotation more info
enables farnesyltranstransferase activity	IEA Inferred from Electronic Annotation more info
enables squalene synthase activity	IBA Inferred from Biological aspect of Ancestor more info
enables squalene synthase activity	IDA Inferred from Direct Assay more info	PubMed
enables squalene synthase activity	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in ergosterol biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in ergosterol biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in ergosterol biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in farnesyl diphosphate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in isoprenoid biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in lipid biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in sterol biosynthetic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in endoplasmic reticulum	HDA more info	PubMed
is_active_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
is_active_in endoplasmic reticulum membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
located_in membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrial outer membrane	HDA more info	PubMed
located_in mitochondrion	HDA more info	PubMed

General protein information

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Preferred Names: bifunctional farnesyl-diphosphate farnesyltransferase/squalene synthase

NP_012060.1

EC 2.5.1.21

Farnesyl-diphosphate farnesyl transferase (squalene synthase); joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001140.6 Reference assembly

Range

484845..486179

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179321.1 → NP_012060.1 TPA: bifunctional farnesyl-diphosphate farnesyltransferase/squalene synthase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012060.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DLD8, P29704

UniProtKB/TrEMBL

A0A8H4F9P6, A6ZT96, B5VKB8, C7GRL2, C8Z9Y9, E9P9H4, N1P4W6

Conserved Domains (1) summary

TIGR01559
Location:37 → 377

squal_synth; farnesyl-diphosphate farnesyltransferase