Physical interaction between interleukin-12 receptor beta 2 subunit and Jak2 tyrosine kinase: Jak2 associates with cytoplasmic membrane-proximal region of interleukin-12 receptor beta 2 via amino-terminus

Biochem Biophys Res Commun. 1999 Apr 13;257(2):400-4. doi: 10.1006/bbrc.1999.0479.

Abstract

IL-12 is a heterodimeric cytokine, composed of p40 and p35 subunits, that exerts its biological effects by binding to specific cell surface receptors. Two human IL-12 receptor proteins, designated IL-12R beta 1 and IL-12R beta 2, have been previously identified. IL-12R beta 2 has box 1 motif, box 2 motif, and three tyrosine residues in its cytoplasmic domain. In response to IL-12, Jak2 and Tyk2, family members of Janus family protein tyrosine kinases, are phosphorylated in PHA-activated T lymphocytes. The present study demonstrates that Jak2 binds to the cytoplasmic membrane-proximal region of IL-12R beta 2, and box 2 motif and tyrosine residues in the cytoplasmic domain were not required for binding. The amino-terminus of Jak2 is necessary for association with IL-12R beta 2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Substitution
  • Animals
  • COS Cells
  • Cell Membrane / metabolism*
  • Cytoplasm / metabolism
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Janus Kinase 2
  • Phosphorylation
  • Phosphotyrosine / metabolism
  • Precipitin Tests
  • Protein Binding
  • Protein-Tyrosine Kinases / chemistry
  • Protein-Tyrosine Kinases / genetics
  • Protein-Tyrosine Kinases / metabolism*
  • Proteins / genetics
  • Proteins / metabolism
  • Proto-Oncogene Proteins*
  • Receptor Protein-Tyrosine Kinases / genetics
  • Receptor Protein-Tyrosine Kinases / metabolism
  • Receptor, EphB4
  • Receptors, Eph Family
  • Receptors, Interleukin / chemistry
  • Receptors, Interleukin / genetics
  • Receptors, Interleukin / metabolism*
  • Receptors, Interleukin-12
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / isolation & purification
  • Recombinant Fusion Proteins / metabolism
  • Sequence Deletion
  • Transfection
  • Tyrosine / genetics
  • Tyrosine / metabolism

Substances

  • Proteins
  • Proto-Oncogene Proteins
  • Receptors, Interleukin
  • Receptors, Interleukin-12
  • Recombinant Fusion Proteins
  • Phosphotyrosine
  • Tyrosine
  • Protein-Tyrosine Kinases
  • Receptor Protein-Tyrosine Kinases
  • Receptor, EphB4
  • Receptors, Eph Family
  • JAK2 protein, human
  • Janus Kinase 2