Abstract
IL-12 is a heterodimeric cytokine, composed of p40 and p35 subunits, that exerts its biological effects by binding to specific cell surface receptors. Two human IL-12 receptor proteins, designated IL-12R beta 1 and IL-12R beta 2, have been previously identified. IL-12R beta 2 has box 1 motif, box 2 motif, and three tyrosine residues in its cytoplasmic domain. In response to IL-12, Jak2 and Tyk2, family members of Janus family protein tyrosine kinases, are phosphorylated in PHA-activated T lymphocytes. The present study demonstrates that Jak2 binds to the cytoplasmic membrane-proximal region of IL-12R beta 2, and box 2 motif and tyrosine residues in the cytoplasmic domain were not required for binding. The amino-terminus of Jak2 is necessary for association with IL-12R beta 2.
Copyright 1999 Academic Press.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Substitution
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Animals
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COS Cells
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Cell Membrane / metabolism*
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Cytoplasm / metabolism
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Janus Kinase 2
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Phosphorylation
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Phosphotyrosine / metabolism
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Precipitin Tests
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Protein Binding
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Protein-Tyrosine Kinases / chemistry
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Protein-Tyrosine Kinases / genetics
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Protein-Tyrosine Kinases / metabolism*
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Proteins / genetics
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Proteins / metabolism
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Proto-Oncogene Proteins*
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Receptor Protein-Tyrosine Kinases / genetics
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Receptor Protein-Tyrosine Kinases / metabolism
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Receptor, EphB4
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Receptors, Eph Family
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Receptors, Interleukin / chemistry
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Receptors, Interleukin / genetics
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Receptors, Interleukin / metabolism*
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Receptors, Interleukin-12
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / isolation & purification
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Recombinant Fusion Proteins / metabolism
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Sequence Deletion
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Transfection
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Tyrosine / genetics
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Tyrosine / metabolism
Substances
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Proteins
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Proto-Oncogene Proteins
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Receptors, Interleukin
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Receptors, Interleukin-12
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Recombinant Fusion Proteins
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Phosphotyrosine
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Tyrosine
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Protein-Tyrosine Kinases
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Receptor Protein-Tyrosine Kinases
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Receptor, EphB4
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Receptors, Eph Family
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JAK2 protein, human
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Janus Kinase 2